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?4C). The most significant categories discovered were lipid catabolic processes (15 genes) and lipid metabolism (36 genes). We validated our results by using qRT-PCR (TLDA) assay, demonstrating that mRNA expression of SerpinE1, SerpinB2, Vim, and CD44 genes were significantly up-regulated in cocultured DA3 TLDA (light-gray bars), DA3 gene profiling array (dark-gray bars) as compared with the mono-cultured DA3 controls (black bars) (Fig.?4D). These four genes are related to wound healing responses, cell migration, biological adhesion, and cell proliferation. Differential gene expression of cocultured PO (MBA-15) cells compared to mono-cultured cells (FC threshold cutoff >2 or FC less than ?2 and P-value? http://www.selleck.cn/products/3-methyladenine.html (Chen et?al. 2009) and GATHER (Gene?Annotation?Tools) (Solinger et?al. 2010) failed to assign significant enriched GO functions. We carried out the GO enrichment analysis on 142 differentially expressed http://www.selleckchem.com/products/r428.html genes (threshold cutoff FC?>?4 or less than ?4 and P-value? http://www.selleckchem.com/products/PF-2341066.html functions. The most statistically significant category was cell junction organization, which included ten genes. Figure?6B and Table?S6 (Supporting Information) demonstrate gene overlap that exists between the various enriched GO categories. Genes whose expression was shared by four or more biological processes are indicated in blue in the table. Figure?6B is a Venn diagram showing five common genes (F11r, Dsp, Jup, Lama5 and Cdh1) among three biological processes: cell junction organization, cell adhesion, and epithelium development. Four genes (Itgb4, Nf2, Cdh3, and Cadm1) are shared by the cell junction and cell adhesion processes. Next, we validated the gene profiling array results by performing qRT-PCR (TLDA) (Fig.?6C). The mRNA expression levels of Lama5, Cadm1, Lamb3, L1cam, Elf3, and Bmp7 were significantly down-regulated in the cocultured MBA-15 TLDA (light-gray bars), MBA-15 gene profiling array (dark-gray bars) in comparison with the basal expression of mono-cultured MBA-15 cells (black bars). Lama5, Cadm1, Lamb3, and L1cam are mainly related to biological adhesion, while Elf3 and Bmp7 are related to cell proliferation and locomotion, according to the enriched GO categories.