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Figure 1 (panels A�CC) shows the mass spectra, obtained in the positive reflectron mode of: an unspiked healthy donor (blank sample; panel A), a healthy donor spiked with ethambutol, pyrazinamide, rifampicin, and streptomycin (quality control sample; panel B), and a TB-infected patient under treatment with ethambutol, pyrazinamide, rifampicin, and streptomycin (panel C). According to literature (11�C14), the protonated molecular ions of ethambutol, pyrazinamide, rifampicin, http://www.selleckchem.com/products/gsk1120212-jtp-74057.html and streptomycin corresponded to 205 m/z, 124 m/z, 823 m/z, and 582 m/z, respectively (Fig. 1, panels B and C). The MS/MS spectra obtained from plasma of a TB-infected patient, treated with ethambutol, pyrazinamide, rifampicin, and streptomycin, are shown in Figure 1 (panels D�CG). The MS/MS spectra obtained from plasma of a TB-infected patient, treated with ethambutol, http://www.selleckchem.com/products/Bortezomib.html pyrazinamide, rifampicin, and streptomycin (Fig. 1, panels D�CG), are in excellent agreement with those from plasma of a healthy donor spiked with anti-TB drugs (MS/MS spectra not shown). Values of protonated fragment ions (MS/MS peaks) of ethambutol, pyrazinamide, rifampicin, and streptomycin (Table 1 and Supporting Information, Figs 1�C3) are in excellent agreement with those reported in the literature (13, 14). This allowed to unequivocally detect ethambutol, pyrazinamide, rifampicin, and streptomycin in the plasma of the TB-infected patient treated with anti-TB drugs. For the concentration to be accepted as the lower limit of quantification (LLOQ), the percent deviation from http://www.selleck.cn/products/bgj398-nvp-bgj398.html the nominal concentration (measure of accuracy) and the relative standard deviation (measure of precision) resulted lower than 20%, according to literature (9, 10). The linearity for ethambutol, pyrazinamide, rifampicin, and streptomycin is satisfactorily described by unweighted least-squares linear regression. The response was linear between LLOQs and 200 pmol/��L for each anti-TB drug (r2 > 0.99). LLOQ values for ethambutol, pyrazinamide, rifampicin, and streptomycin, together with regression data, are reported in Table 2. The recovery rates of the anti-TB drugs ranged between 87 and 110% (data not shown). Intra-day and interday precision and accuracy values, obtained as previously reported, are shown in Table 3. Precision and accuracy values were