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?monocytogenes during frozen storage and completely inhibited its growth during the subsequent storage at 4��C. To our knowledge, this is the first research investigating the effect of long-term frozen storage and edible coatings on the growth of L.?monocytogenes on cold-smoked salmon during subsequent refrigeration storage. Yoon et?al. (2004) investigated the effect of freezing stress (5?days) on the growth kinetics of L.?monocytogenes Scott A on smoked salmon at 4 and 10��C. Freezing stress significantly extended the lag time and http://www.selleckchem.com/products/PD-0325901.html delayed the growth of L.?monocytogenes Scott A at both 4 and 10��C; however, addition of PL (1��85 and 2��8%) combined with SD (0��12 and 2%) completely controlled freeze-stressed cell growth for 30?days. We also observed that when combined with prior frozen storage, the alginate, gelatin and starch plain coatings were able to inhibit the growth of L.?monocytogenes during the subsequent 30-day refrigerated storage. However, in the absence of frozen storage, the plain coatings only had marginal effects. In this study, plain coatings were formulated by dissolving the coating materials in a solvent of 1% acetic acid with a final concentration of 0��06% (w/w) of salmon (=1% acetic acid*0��6?ml of coating solution*/10?g of salmon). The actual concentration of acetic acid might be even lower than 0��06% because some acetic acid probably evaporated during the 45-min drying of the coated cold-smoked salmon. http://www.selleckchem.com/products/3-methyladenine.html To determine whether acetic acid alone had any antilisterial effect, we applied it directly on inoculated cold-smoked salmon http://www.selleck.cn/products/Cisplatin.html at a level of 0��06% (w/w) of salmon. The acetic acid alone had very similar antilisterial effect to the plain coatings with