What is fluorescent labeling?

Fluorescent labeling is the process of covalently attaching fluorescent groups to proteins, nucleic acids and other molecules. Fluorescein group derivatives that can selectively react with functional groups present on the target molecule are usually used to complete such a process. Common labeling molecules are antibodies that often use labeled antibodies to detect specific target molecules. Fluorescent labels can be used in a variety of detection systems to achieve sensitive and quantitative detection.

The nerve fiber endings of Fluorescent labeling can absorb a lot of fluorescent compounds or fluorescent dyes, which are transported back into the cell body through axons, thereby establishing a retrograde fluorescent labeling method.

Fluorescent labeling technology

The compound used for fluorescent label is called a fluorescent substance. Fluorescent substance refers to a compound with a chemical structure of a conjugated double bond system. When irradiated by ultraviolet light or blue-violet light, it can be excited into an excited state, and when it returns to the ground state from the excited state, it emits fluorescence.

Fluorescent labeling technology refers to the use of fluorescent substances to covalently bind or physically adsorb on a certain group of the molecule to be studied, and use its fluorescent properties to provide information about the object under study. The advantages of fluorescent labeling, such as no radioactive pollution and easy operation, make the application of fluorescent labeling in many research fields increasingly widespread.

Fluorescent markers are also widely used in the fields of protein function research and drug screening. Researchers use fluorescently labeled peptides to detect the activity of target proteins, and the high-throughput activity screening methods developed by them are applied to drug screening and drug development of disease treatment target proteins (for example, various kinases, phosphatases, peptidases) Wait). Therefore, the fluorescent modification of peptides is also an important content in the field of peptide synthesis.

1. FITC modification

Fluorescein isothiocyanate (FITC) has relatively high activity. Generally speaking, it is easier to introduce this type of fluorophore during solid-phase synthesis than other fluoresceins, and there is no need to add activating reagents during the reaction.

1. AMC modification

7-Amino-4-methylcoumarin (AMC) is a widely used fluorescent labeling reagent, for example, trace determination of enzymes, identification of enzymes, preparation of laser dyes and many other applications. In addition, the C-terminal Coumarin-modified ubiquitin molecules are also important probes for studying the process of protein ubiquitination.

Different from other fluorescent dyes, AMC modified peptide molecules are carried out from the C-terminus: (1) AMC reacts with the first amino acid at the C-terminus of the peptide chain; (2) the solid phase synthesis of the entire peptide chain (starting from the second amino acid) ), and retain the side chain protecting group and the last amino protecting group of the entire peptide chain; (3) Liquid phase condensation of AA-AMC and fully protected peptide chain; (4) Removal of the protecting group to complete the modification of the peptide chain.

About Boc Sciences

BOC Sciences not only provides the synthesis of small molecules and biopolymers, but also contributes to functionalization or activation of compounds ready for cross-linking with pre-activated small molecules. These small molecules are either supplied by customers or prepared internally. After labeling biopolymers with small molecules, the standard desalination, purification, quality inspection, final concentration and labeling rate were determined. BOC Sciences specializes in synthesizing new small molecules, modifying existing molecules, and advising customers on appropriate ways to activate small molecules, label small molecules, and combine small molecules with biomolecules. As one of the pioneers of synthetic biological services, we are actively seeking to develop new technologies to help customers develop new biological binding reagents for sensitive testing applications. BOC Sciences is committed to supplying cost-effective products and services, which are being utilized by our customers worldwide in drug discovery and research on a daily basis to promote innovations in various industries.