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0?kDa) (Anawa Trading, Wangen, Switzerland). http://www.selleckchem.com/products/Rapamycin.html The following prestained standard protein molecular weight markers were used: phosphorylase b (97.4?kDa), bovine serum albumin (66.2?kDa), l-glutamic dehydrogenase (55.0?kDa) and ovalbumin (42.7?kDa) (all from Promega, Madison, Wisconsin, USA). Gelatinolytic activity was quantified using a computer-assisted image analysis program (1D Image Analysis Software, Kodak Digital Science v. 3.0; Eastman Kodak, Rochester, New York, USA). The Bradford assay (Bio-Rad, Glattbrugg, Switzerland) employing bovine serum albumin (Sigma-Aldrich Chemie, Steinheim, Germany) as standard was used in order to determine protein content in SRF and vitreous fluid aliquots. All data were normalized per protein content for each sample. Data are presented as mean?��?standard error (SE) values. Data between patient and control groups as well as between subgroups of the patient group were compared with the use of Student��s independent samples t-test and analysis of variance. Determination of possible correlations between MMP and TIMP-1 levels and PVR grade was performed using the Spearman rank correlation test. Regression http://www.selleck.cn/products/Bleomycin-sulfate.html analysis was used to introduce a possible MMP model that correlated with PVR grade. Differences were considered statistically significant at a p-value http://www.selleckchem.com/products/MG132.html patients exhibiting PVR grade A, 21 grade B and six grade C. In the vitreous group, PVR was identified in 24 of 32 patients (75%), with three patients exhibiting PVR grade A, 11 grade B and 10 grade C. MMP and TIMP-1 concentrations in subretinal fluid and vitreous of PVR patients and in vitreous fluid of eyes of organ donors (control group) are shown in Table 1. Graphical representation of the results revealed that, in SRF, peak MMP-1 (Fig.?2A), -3 (Fig.?2D), -8 (Fig.?2E), proMMP-9 (Fig.?2F) and TIMP-1 levels (Fig.?2H) coincided with PVR grade B, and that of proMMP-2 (Fig.?2B), MMP-2 (Fig.?2C) and MMP-9 (Fig.?2G) coincided with PVR grade C (Fig.?1). Peak values were statistically significant for proMMP-9 (p?=?0.012), active MMP-1 (p?=?0.011), MMP-2 (p?=?0.05), pro- and active MMP-3 (p?=?0.033), pro- and active MMP-8 (p?=?0.018), MMP-9 (p?=?0.05) and TIMP-1 (p?=?0.02), compared to lower and/or greater PVR grades (Table?2). Correspondingly, in vitreous, highest MMP-1 (Fig.?2A), proMMP-2 (Fig.?2B), -9 (Fig.?2F) and MMP-9 levels (Fig.?2G) coincided with PVR grade B, and that of MMP-2 (Fig.?2C), -3 (Fig.?2D), -8 (Fig.?2E) and TIMP-1 (Fig.?2H) coincided with PVR grade C (Fig.?1). Observed peak values were statistically significant for active MMP-1 (p?=?0.007), proMMP-2 (p?=?0.011), MMP-2 (p?=?0.005), pro- and active MMP-3 (p?= 0.013), pro- and active MMP-8 (p?=?0.017), proMMP-9 (p?=?0.008), MMP-9 (p?=?0.012) and TIMP-1 (p?=?0.