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The thickness was measured from the middle part of the image with a calibration slide (Carl Zeiss, Oberkochen, Germany). Pilot tests had shown that there was no difference of thickness before and after the experiments (data not shown). Diffusion from the donor chamber to the receiver chamber was characterized by means of a permeability constant Pc (cm/s). The cumulative amount of a permeant transported across the sclera was plotted against time (accumulative�Ctime curve). The steady-state flux of the permeant was calculated from the slope of the linear regression of the linear region in the plot divided http://www.selleckchem.com/products/bms-911543.html by the effective diffusion area and the initial concentration of the solution (the change of concentration of the initial solution (C0) must be http://www.selleck.cn/products/ON-01910.html initial concentration of the solution. The photographs were changed to black and white pictures (Photoshop CS4), and the thickness of the area that macromolecules enriched in the ��outer�� sclera surface (surface that facing up during the experiment) at geographical centre was measure with a calibration slide (Carl Zeiss, Oberkochen, Germany). The thickness was determined as the distance from the scleral surface to the depth where grey scales fell down to a half of the maximum. For each picture, the measurement was performed at least for 5 times. Mann�CWhitney U-test was used to compare Pc between different topographical locations of the sclera for each FITC-compound. Statistical analysis of Pc of different molecular weight FITC-compounds at the same location, thickness among different scleral locations and thickness of area that macromolecules enriched in the surface of the sclera over different locations for each tracer were performed with Kruskal�CWallis test followed by the Mann�CWhitney U-test (spss, ver 17.0; SPSS, Chicago, IL, USA). Statistical significance was set at p? http://www.selleckchem.com/products/loxo-101.html of anterior, equatorial and posterior superotemporal sclera (n?=?6 for each measurement) were 0.347?��?0.042?mm, 0.662?�� 0.153?mm and 0.865?��?0.090?mm, respectively. The average thickness of porcine sclera measured at anterior, equatorial and posterior superotemporal varied significantly (Kruskal�CWallis, p?