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Noll & Wellinger (2008) were able to distinguish the young and old soil subdivisions on 16S rRNA gene-based fingerprints by incorporating more samples for each subdivision. Different levels of inorganic sulfate in young and old soil (Table 1), respectively, had no significant effect on the structure of asfA gene-based fingerprint patterns (F-ratio=1.088, P=0.325) as well as on the corresponding 16S rRNA gene-based fingerprint patterns (F-ratio=0.894, P=0.092.). The rhizosphere analysis was carried out with samples from the pioneering plant species L. alpina and A. rupestris harvested http://www.selleckchem.com/products/PLX-4032.html from young and old soil, respectively. Desulfonating communities of the A. rupestris rhizosphere and the old soil communities differed significantly (F-ratio=1.305, P=0.0117, and F-ratio=1.407, P=0.0033 for A. rupestris from young and old soil, respectively; Fig. 2), http://www.selleckchem.com/products/INCB18424.html and clear (but not significant) separations were observed between the A. rupestris rhizosphere and the young soil (F-ratio=1.128, P=0.0615, and F-ratio=1.162, P=0.0892 for A. rupestris from young and old soil, respectively; Fig. 2). Furthermore, the desulfonating community in the A. rupestris rhizosphere in the old soil was significantly different when compared with the surrounding old soil itself (F-ratio=1.407, P=0.0033) and a similar (but not significant) trend existed in the young soil (A. rupestris in young soil vs. young soil, F-ratio=1.162, P=0.0615; Fig. 2). In contrast, the desulfonating rhizobacteria communities in L. alpina harvested from old and young soil, respectively, were not significantly different (F-ratio=0.913, P=0.772) and grouped closely with those from the old soil (e.g. were not significantly different. F-ratio=1.120, P=0.0813; Fig. 2). Ordination graphs of the 16S rRNA gene fingerprint patterns of A. rupestris and L. alpina rhizospheres indicated http://www.selleck.cn/products/U0126.html significant differences from the ones from the soils (F-ratio=2.952, P=0.0001, Fig. 1). Despite the proximity in the ordination graphs (Fig. 1), significant differences were detected between the two plant host communities (F-ratio=2.807, P=0.0085). However, the soil age had no significant effect on the structure of the rhizobacterial community in both plant host species (L. alpina: F-ratio=1.417, P=0.097; A. rupestris: F-ratio=1.219, P=0.098; Fig. 1). Concentrations of inorganic sulfate in the rhizospheres of A. rupestris from old and young soil and L. alpina from old and young soil (8.3�C14.6?��g?sulfate-S?g?1 root fresh weight, Table 1) were substantially higher when compared with the results from the bulk soil analysis (