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?azaleae (AB182266), M.?baccarum (Z73773), M.?cassiopes (Z73776), M. fructicola (Z73777), M.?fructigena (Z73781), M.?gaylussaciae (Z73782), M.?jezoensis (AB182265), M.?johnsonii (Z73783), M.?laxa (Z73786), M.?mali (AB125615), M.? megalospora (Z73788), http://www.selleckchem.com/products/XL184.html M.?mumecola (AB125618), M.?oxycocci (Z73789), M.?padi (Z73791), M.?polycodii (Z73782), M.?polystroma (Y17876), M.?seaverii (Z73793), M.?urnula (Z73794), M.?vaccinii-corymbosi (Z73796), Ovulinia azaleae (Z73797) and Sclerotinia sclerotiorum (Z73783) (Holst-Jensen et?al., 1997; Harada et?al., 2005; Takahashi et?al., 2005). The 5��8S-ITS region sequences were aligned using the computer program mega v4��0 (Tamura et?al., 2007). To obtain appropriate substitution models for Maximum Likelihood (ML) analysis (Felsenstein, 1981), the alignment was analysed with jModelTest 0��1��1 using the Akaike information criterion (AIC) (Posada, 2008). Searches for the best ML tree and 1000 bootstrap replications were done with the fast likelihood software phyml 3��0, using identical settings. Completely gold-yellow ripe fruit of cv. Com��n collected during autumn were inoculated with two isolates of M.?linhartiana (MON-08 and MON-09), two isolates of M.?laxa (MON-01 and MON-02) or two http://www.selleck.cn/products/MLN8237.html isolates of M.?fructigena (MON-04 and MON-05) (Table?1). Before inoculation, the fruit were washed and then surface sterilized by immersing them in a 10% solution of commercial NaClO (50?g?Cl?L?1). Surface sterilized fruit were inoculated by placing a 9?mm diameter PDA plug of mycelium of each isolate on them. Control fruit were treated with a sterile PDA plug. Control and inoculated fruit were placed in plastic moisture chambers (41?��?26?��?20?cm) at 100% RH and 22?��?2��C under fluorescent lights (350?��mol?m?2?s?1). There were five fruit per isolate and the experiment was repeated three times. Fruit were examined weekly for disease symptoms, and lesions that formed around the agar plugs were measured after 1?month. The effects of species and isolate on lesion diameter were determined by an anova and means were compared by the Fisher��s Protected http://www.selleckchem.com/products/CP-690550.html LSD test at P?=?0��05. To study whether the fungus infects the developing fruit, a total of 10 aborted fruit (