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Four measurements were obtained automatically by the Neurolucida software from a constructed CA3 pyramidal neuron, including somal area, and total lengths, branching nodes (or points) and spine density (per 10 ��m dendritic length) of the apical and basal dendrites. A total of 20 hippocampal CA3 pyramidal neurons in each case (10 neurons per hemisphere) were subjected for the above analyses. Means and standard derivations were calculated for each set of data and compared statistically using one-way ANOVA with Bonferroni posttests (GraphPad Prism 4.02, GraphPad Software). The level for significant statistic difference was set at P http://www.selleckchem.com/products/DAPT-GSI-IX.html number of impregated cells were found in CA3 area (Fig. 1A,B). They were relatively small in size, and were round, oval, or fusiform in shape. They had no or a few but short processes. Dendritic spines were rarely identifiable on the labeled cells. We did not perform dendritic morphometry for these two cases because (1) their dendritic structures (branches and spines) were not evident microscopically; and (2) the Neurolucida analysis failed to obtain sufficient dendritic measurements for a systematic comparison with other cases. In the 26 GW hippocampus, impregated CA3 cells seemed slightly larger in somal size http://www.selleckchem.com/products/Gefitinib.html relative to 19 and 20 GM (Fig. 1A�CC). The somata of the labeled cells were mostly triangular or pyramidal. A relatively long apical http://en.wikipedia.org/wiki/NK_cells dendrite and a few short basal dendrites were seen on many of the labeled somata, both having a few orders of branches (Fig. 1C, arrowhead). Spine structures were found on the somata as well as the apical and basal dendrites at high magnifications (Fig. 2A�CC). In the hippocampi from the 35 GW, 38 GW, and 8 Y cases, a large number of CA3 pyramidal cells were stained, with apical and basal dendrites clearly seen but extended over long distances in the strata radiatum, lucidum, and oriens (Fig. 1D�CF). The apical dendrites were considerably thick and had several orders of branches over the stratum radiatum (s.r.) and s.l. (Fig. 1E,G). Basal dendrites arose from the low portion of the somata, and were relatively thin, ranged from two to several and distributed in the strata pyramidale and oriens, some of which gave rise to multiple branches (Fig. 1G,H). An axon or axon-like process could be identified on some pyramidal cells, often arising from the midpoint of the somal base (Fig. 1G).