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Thirdly, the patient had to answer the questions totally individually. The treating dentist could not give any verbal information about the disease or treatment before completing the three tests. During watching and answering, only the dental assistant was present in the consultation room and could only help the patients for technical problems with the computer program. This ensured that the pre-test was completed based on the prior knowledge before entering the practice and potential gain of knowledge http://www.selleckchem.com/GSK-3.html in the post-test and follow-up test would be as a result of the information given in the educational videos. Furthermore, the patients were asked not to guess when they did not have any idea of the answer. In this case, they could click on answer D, ��I don't know��. At last, the information was not given with the patients at home, so they could not re-watch the video before the follow-up test had took place. The null hypothesis was that the AN-group and the CO-group were equal in knowledge evolution after periodontal patient education at the time of the post-test and follow-up test. Age was compared between groups using Anova. The normal quantile plot showed that the basic Anova assumptions were met. Sex and education level were compared using a Fisher's http://en.wikipedia.org/wiki/VAV2 Exact test. Concerning the sum of the 10 questions, the groups were compared with a Wilcoxon signed rank test. The obtained p-values were corrected for simultaneous hypothesis testing according to Sidak. A total of 68 patients participated in this trial. One person of the AN-group could not be reached in time for completing the follow-up test. The results of the three tests were available for analysis from 33 participants in the AN-group and 34 in the CO-group. A detailed study flow chart http://www.selleckchem.com/screening/tyrosine-kinase-inhibitor-library.html is presented in Fig.?2. No harms befell any participant as a result of participation in the trial. The demographic characteristics of both groups, including age, gender and education level, are presented in Table?2. The average age of the patients was not significantly different between both groups (p?=?0.72). Slightly more woman participated in the study (AN-group?=?51.5% and CO-group = 61.8%), however, statistically there was no difference found between both groups (p?=?0.41). Concerning pre-existing knowledge, no statistically significant differences were observed between both groups at baseline level (Table?3). Although the knowledge scores improved in both groups after the periodontal patient education, the scores for the post-test and follow-up test were significantly higher in the AN-group compared with the CO-group (Table?3). Analysing the changes in knowledge recall; (post-follow-up): ��AN =?0.9 and ��CO?=?2.2 (p?=?0.03), there was significantly less recall of knowledge after 2?weeks in the CO-group. Similar with (pre-follow-up): ��AN?=? ?3.8 and ��CO?=??1.3 (p