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The 590/520 ratio of JC-1 (Fig. 5a, subsphere, 0.64 �� 0.07; monolayer, 0.33 �� 0.01; n = 6 �� 104, p http://www.selleckchem.com/products/hydroxychloroquine-sulfate.html during the first 12 hr. After the ����m plateaued between 12 hr and 2 day, the ����m decreased rapidly and reached the levels of the A549 cells (50.8; n = 2 �� 104, Fig. 5c) 4 weeks after induction. The ����m in SP and non-SP A549 cells was also determined using LCM. A higher 590/520 ratio of JC-1 (Figs. 5d and 5e, SP, 1.04 �� 0.13; non-SP, 0.82 �� 0.09; n = 15, p http://www.selleckchem.com/products/blz945.html (sphere, 24 hr, 1.87 mmol/L; 48 hr, 6.11 mmol/L; monolayer, 24 hr, 4.81 mmol/L, 48 hr, 10.76 mmol/L; cell line, 24 hr, 1.06 mmol/L; 48 hr, 9.70 mmol/L) or DMEM containing 10% FBS (cell line, 24 hr, 4.30 mmol/L; 48 hr, 13.01 mmol/L) are presented in Figure 6a. The LCSC subpopulation consumed less glucose than their differentiated descendants. In addition to their lower glucose consumption, these LCSCs also utilized less oxygen (Fig. 6b, sphere, 1.46 �� 0.16; monolayer, 2.52 �� 0.72; n = 3, p http://www.selleck.cn/products/BafilomycinA1.html �� 0.92 ��mol/L/mg; cell line, 3.86 �� 1.73 ��mol/L/mg; n = 3, p