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Individual was included as a random effect to account for non-independence among repeated measures. Interaction terms were omitted because of the relatively small sample size, but intake level was nested within hay type, which serves as a validation check for interaction effects. Where necessary, multiple comparisons were made using Bonferroni post hoc tests. Correlations between individual measurements http://www.selleckchem.com/products/BIBW2992.html were performed either using correlation analysis or using linear regression; 95% confidence intervals (95% CI) are given for coefficients of regression equations. Additional general linear models were used to investigate differences between the two hays for relationships of continuous characters. Subjectively, it appeared that the ponies ingested high-quality hay 1 faster than low-quality hay 2; this appeared to be due to selective feeding behaviour on hay 2, as evident during the ad libitum feeding (Table?1). Even on lower food intake levels, when mostly http://en.wikipedia.org/wiki/SWAP70 all the hay offered was consumed, the ponies appeared to first select the finer parts of hay 2, even interrupting their feeding bouts, before consuming the tougher parts. Ponies lost body mass and body condition with decreasing intake level (Table?2). For the lowest intake level, the equation of Zeyner and Kienzle (2002) overestimated the measured intake of digestible energy on hay 1 (Table?2). A linear regression of the change in body mass on DE intake (y?=?0.538 [95% CI 0.385�C0.691] x ? 0.376 [?0.458 to ?0.294], R2?=?0.63, p? http://www.selleckchem.com/products/BKM-120.html of 40%) and a long particle retention time (37?h vs. an average of 29?h in the other animals, cf. Table?3), and the excretion curve of the particle marker indicated re-ingestion of faeces. Therefore, measurements from this animal on this hay and intake level were excluded from further analyses. Additionally, it was noted that all ponies had increasingly negative calculated digestibility coefficients for acid-insoluble ash (Table?4) with decreasing intake level. Although soil consumption was not specifically noted, this most likely indicates allophagic behaviour during food deprivation. The mean retention time of both the solute and the particle marker generally increased with decreasing food intake level (Table?3). Marker excretion curves showed a later peak and a slower reduction in faecal marker levels with lower food intake levels (Fig.?1). Particle MRT (y) decreased with relative dry matter intake (x in g/kg0.75/day) according to y?=?198 [97�C406] x?0.47 [?0.65 to ?0.30] (R2?=?0.