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Vessel density was measured by superimposing a grid (72 �� 72 ��m grid squares) over each field of view and counting the number of vessel segments that touched each vertical line on the grid. Vessels were marked and counted using the Cell Counter plug-in feature of ImageJ. Vessel length-density was determined by tracing vessels falling within in each field of view and measuring the total length of vessel segments using the NeuronJ plug-in feature of ImageJ. Mean vessel diameter per field of view was obtained by averaging the diameter measurements of five randomly selected vessels within each field of view in embryonic and abembryonic regions of the uterus. Statistical analyses were conducted using SAS Statistical Package http://www.selleckchem.com/screening/epigenetics-compound-library.html version 9.1.2 (SAS Institute,2004). Contrasts of microvascular density and http://www.selleckchem.com/products/Dasatinib.html surface-area (Nv, Lv, and Dv) between species and among embryo stages were evaluated using a two-factor analysis of variance (ANOVA). For C. taeniolatus, the effect of reproductive condition on uterine Nv, Lv, and Dv was analyzed using a Student's t-test. For S. equalis, the effect of embryo stage on uterine Nv, Lv, and Dv was analyzed using a single factor ANOVA. Analyses of the effect of embryo stage on uterine vascular proliferation were based upon the average of data obtained from three fields of view for each embryonic and abembryonic region of the uterus. The effect of embryo stage on chorioallantoic vascular proliferation was based on the average of data from three fields of view obtained from each chorioallantoic membrane. Data are reported as mean �� SE unless otherwise noted https://en.wikipedia.org/wiki/Evodiamine and probability values less than 0.05 were considered statistically significant. In C. taeniolatus, there is no significant variation in overall Nv, Lv, or Dv during the period of gravidity (Table 1; Figs. 2A,C,E, 3A,C,E,G). There is correspondingly little change in embryo mass during the period of egg retention, with an overall increase in dry mass of 0.37 mg from early developmental stages (