The Latest Pointers Around SRT1720 Never Before Unveiled

4 �� 0.9 ��m; Ang1-9, 26.3 �� 0.8 ��m; Ang1-9 + captopril, 24.8 �� 0.8 ��m; P and that it has anti-hypertrophic effects in cardiomyocytes. Ang1-7 has been described to antagonise AngII-induced hypertrophy by engaging the Mas receptor (Santos et al. 2003, 2006; Dias-Peixoto et al. 2008; Pinheiro et al. 2009). To determine whether Ang1-9 signalled via Mas we used the specific Mas antagonist A779. Pre-incubation of AngII-stimulated H9c2 cardiomyocytes with 10 ��m A779 prior to the addition of Ang1-7 blocked its anti-hypertrophic effect (non-stimulated, 191.1 �� 6.6 ��m; AngII-stimulated, 288.6 �� 12.3 ��m; Ang1-7, 218.3 �� 9.8 ��m; Ang1-7 + A779, 275.5 �� 14.0 ��m; P effects of Ang1-7, without affecting the actions of Ang1-9 (Fig. 3B). These results confirmed that Ang1-9 has an independent effect on cardiomyocytes from that of Ang1-7 and that it signals via a different receptor from Mas. Next, to determine which receptor Ang1-9 was signalling via we blocked the AT1R with losartan (Fig. 4). As AngII signals through the AT1R, we induced hypertrophy with arginine vasopressin. First, we confirmed that pre-incubation with losartan blocked AngII-induced hypertrophy (Fig. 4). Arginine vasopressin, however, was still able to induce cardiomyocyte hypertrophy in the presence of either 1 or 10 ��m losartan (non-stimulated, 179.2 �� 5.4 ��m; AngII-stimulated, 253.4 �� 8.4 ��m; AngII + losartan 1 ��m, 201.0 �� 6.1 ��m; Arg-vasopressin, 227.4 �� 9.8 ��m; Arg-vasopressin + losartan 1 ��m, 247.8 �� 7.2 ��m; P we confirmed that AT2R agonism blocked AngII induced hypertrophy using the selective agonist CGP42112 (Ohkubo et al. 1997) (Supplemental Fig. S1). To assess the role of the AT2R in the effects of Ang1-7 and Ang1-9, the AT2R antagonist PD123,319 was utilised. Addition of PD123,319 at 100 nm, 500 nm or 1 ��m alone did not affect AngII-induced hypertrophy, or result in inhibition of either ANP or BNP expression, confirming that AngII-induced hypertrophy in this model was via the AT1R and did not involve the AT2R (Fig.