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At the two last fishing events, a sub-sample of eels not marked with CWTs was killed and stored for further investigations, as described above, to determine the size distribution of each stocking batch (Table?3). Sagittal otoliths of eels not marked with CWTs were extracted, cleaned and stored in 96% ethanol. One otolith per individual was embedded with the convex side up in transparent wax (Mounting Wax Crystalbond 509; Fa. Buehler GmbH, D��sseldorf, Germany) on a microscope slide and ground with a series of grinding papers (600, 800 and 1200 grade) down to the centre. Finally, the otolith was observed under a UV-light microscope (Fa. Ernst Leitz Wetzlar GmbH, Wetzlar, Germany) equipped with two epifluorescence filters, one of 515�C560-nm wavelength and one of 355�C425-nm wavelength at 125-fold magnification to identify ARS or OTC marks. In all cases, marks were clearly identifiable http://www.selleckchem.com/products/PLX-4032.html as mark. Eels showing no ARS or OTC marks on the otoliths were excluded from the sample as not belonging to the stocked population. Only individuals identified as originally stocked eels were included in the analysis (Table?3). For population size estimation in 2010, stocking batches and resident eels in each lake were identified with the help of subsamples investigated in the laboratory and by length frequency analyses of the capture http://www.selleck.cn/products/BEZ235.html protocols with the Bhattacharya method (Bhattacharya 1967), using FiSAT II (Gayanilo et?al. 1995). Population size of stocked eel batches was estimated with Bailey's (1951, 1952) modification of the Lincoln�CPetersen estimation by the last fishing occasions and with the Schnabel (1938) estimator as described by Krebs (1999). Estimates with one or no recaptured marked eel and for which the coefficient http://www.selleckchem.com/products/AZD2281(Olaparib).html of variation was larger than 0.5 were excluded because such estimates have a too low precision. To estimate the percentage of eels surviving from each stocking batch, the population size of eels estimated in 2010 was set in relationship to the number of eels stocked initially. Statistical analyses were performed with spss 9.0. (SPSS Inc., Chicago, IL, USA). Because the assumptions of normality and homogeneity of variance were not met, nonparametric Mann-Whitney-tests (U-test) were used to test for significant differences between mean LT of the recaptured eels stocked as glass eels or farm eels at stocking and in each year of recapture in each lake. The minimum sample size was set at six, and the significance level was taken as P?