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2001; Ribera & Vogler 2004). Contrasts were produced using the CRUNCH algorithm of the CAIC software package (Purvis & Rambaut 1995), and regressions of contrast scores forced through the origin (Garland, Harvey & Ives 1992). Species�� southern range limits were normally distributed, whilst latitudinal range extent and central point were normalized following log10 transformation. http://www.selleckchem.com/products/Vorinostat-saha.html In the case of northern range limits, data were normalized following double log10 transformation. Normality in all cases was assessed via Shapiro�CWilks test; P?>?0��05. All statistical analyses were conducted using JMP IN? version 5.1, except for multiple regression models, which were run in R v.2.5.1 (R Development Core Team, 2007) http://www.selleck.cn/products/ve-821.html and SPSS v.15.0. Upper and lower thermal limits differed significantly between species of Deronectes at all acclimations tested (ANCOVA minimum F13,173?=?8��797; P? http://www.selleckchem.com/products/Cisplatin.html Supporting Information; Fig.?1a), and mean LTL ranged from ?3��4?��C in D. algibensis Fery and Fresneda, to ?10��0?��C in D. latus, both following acclimation at 14��5?��C (Appendix?S2, Supporting Information; Fig.?1b). Furthermore, phylogenetically independent contrasts reveal that the ability to tolerate heat and cold are significantly negatively correlated across the genus (Pearson correlation Z11?=?3��779; P?=?0��0004). Mean ��UTL ranged from ?1��13?��C in D. wewalkai Fery and Fresneda to 2��02?��C D. latus, whilst mean ��LTL ranged from ?1��99?��C in D. opatrinus to 1��42?��C in D. platynotus mazzoldi Fery and Brancucci (see Appendix?S2, Supporting Information). Phylogenetically independent contrasts reveal that the ability to acclimate to heat and cold are significantly positively correlated across the genus (Pearson correlation Z11?=?7��679; P?