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The averages of Sobs, Sest (species plot?1) and individual densities (individuals?ha?1) were 38.5?��?6.8, 49?��?8.6 and 1742.3?��?522.1, respectively, in forest type 1 (subtropical evergreen broadleaved forests); 73.2?��?17.9, 90.2?��?22.6 and 2805.0?��?461.3, respectively, in forest type 2 (monsoon evergreen broadleaved forests); and 142.0?��?72.7, 214.0?��?99.7 and 2615.0?��?49.8, respectively, in forest type 3 (tropical forests). The estimated missed species (Sest�CSobs) due to insufficient plot size are 17�C23% of Sest for type 1 and 2 and 30�C38% of Sest for type 3. Both the observed species richness (Sobs, species plot?1) shown in Table?1 and the observed individual densities (individuals?ha?1) depend on the forest types but not on plot sizes. Correlation analysis pointed out that the relationship between plot size and Sobs or observed individual densities (individuals?ha?1) for all plots http://www.selleckchem.com/products/Nutlin-3.html were not statistically significant. Variance analysis also showed that both Sobs and individual densities (individuals?ha?1) in forest type 1 were significantly different http://www.selleck.cn/products/Methazolastone.html from those in forest type 2 and 3. The plot sequence in accordance with Sest (species plot?1) was exactly the same as that in accordance with Sobs (species plot?1) (Table?1), which shows that the observed species richness and individual features from the thirteen plots well characterized the forests that the plots represented, and all plots met the requirement for performance areas of different forest types. Among the thirteen permanent plots in China's tropical/subtropical biome, marked temporal changes were observed in the structure of the stands, the functional dominance of individuals, and the individual species of native vegetation (Figs?2-4). Structurally, the number of individuals in the smaller DBH classes (I and II) and the functional groups representing small statured vegetation (shrub and small tree) significantly increased (P??0.085). Likewise, the total number of individuals in each plot and all the thirteen plots combined significantly increased over the period (P? http://www.selleckchem.com/products/ABT-737.html (Fig.?2). The percentage of individual numbers in the two smaller DBH classes increased from 76% to 89%, with those in DBH class I increasing from 48% to 64%, whereas those in the DBH class II decreasing from 28% to 25%. The percentage of individual numbers in small tree and shrub classes increased from 10% to 37% and 3% to 17%, respectively, and the total number of individuals increased 93%. Table S1 shows the species with significant changes of individual number in the three permanent plots, HTK, DH1, and BN2, which represent the three main types of China's tropical and subtropical forests, subtropical evergreen broadleaved forest, monsoon evergreen broadleaved forests, and tropical forests, respectively.