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To establish whether other major plasma androgens (other than T) are present in ground squirrel plasma, we analysed a number of samples with highly specific commercial antibodies. As little plasma remained from many of the samples used in these four studies, we analysed additional representative samples. To assess the DHT levels, we used a RIA kit (DSL-9600; Diagnostic Systems Laboratories, Webster, TX, USA) with the antibody having cross-reactivities of 0��2% to T and 1��9% to androstenedione. All samples listed below were analysed in a single assay, and thus, there was no inter-assay variation. The kit intra-assay coefficient of variation was 0��29?ng?mL?1). We found that DHT levels in AGS breeding males (mean?��?1 SE: 1��53?ng?mL?1?��?0��38, N?=?15), nonbreeding, pre-hibernation males (1��28?��?0��18, N?=?10), and nonbreeding, pre-hibernation females (1��09?��?0��239, N?=?6) tended to be higher than those http://www.selleckchem.com/products/AZD6244.html in pregnant (0��46?��?0��14, N?=?6) and lactating females (0��15?��?0��03, N?=?3) (F4,35?=?2��58, P?= 0��054). In CGS, DHT levels were uniformly low and about 16�C20% of those in comparable classes in AGS (breeding males 0��24?��?0��02, N?=?11; nonbreeding, pre-hibernation http://www.selleck.cn/products/Imatinib(STI571).html males 0��24?��?0��01, N?=?10, and nonbreeding, pre-hibernation females 0��22?��?0��10, N?=?14). Thus, plasma levels of DHT in AGS may account for up to 20% of androgen levels in breeding males and pre-hibernation males and females (Figs?2 and 3). To assess the androstenedione levels, we used an enzyme immunoassay kit (DRG EIA-3265; DRG International, Marburg, Germany) with the antibody having cross-reactivities of 0��1% to T and 2��6?ng mL?1). In AGS, androstenedione levels in breeding males (4��46?ng?mL?1?�� 0��55, N?=?10), nonbreeding, pre-hibernation males (4��60?��?0��39, N?=?24), and nonbreeding, pre-hibernation females (3��14?��?0��60, N?=?17) http://www.selleckchem.com/products/SP600125.html were significantly higher than those in pregnant (0��46?�� 0��141, N?=?6) and lactating females (0��38?��?0��13, N?=?6) (F4,58?= 10��57, P?