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The mean age of patients with multiple viral findings was 12.7?months, compared to 5.7?months for those with single findings (p? http://www.selleckchem.com/products/BIBF1120.html older than those with a single detected virus (193 episodes), with mean ages of 12.7 and 5.7?months, respectively. No other major differences in the presentation or clinical outcome were observed between the two groups. Acute respiratory infections are the most common cause of morbidity and hospitalisation in children. The diagnostic methods have greatly improved by new molecular techniques. The most common respiratory viruses found in hospitalised children under two years of age are respiratory syncytial virus (RSV) and influenza virus A and B [1, 2]. http://www.selleckchem.com/products/Metformin-hydrochloride(Glucophage).html Parainfluenzavirus 1-3 (PIV), rhinovirus and adenovirus are also important pathogens among children with respiratory infections [3, 4]. During recent years, at least five new pathogens causing respiratory disease have been described: human metapneumovirus (hMPV), three new coronaviruses (HCoV-NL63, HCoV-HKU1 http://www.selleck.cn/products/carfilzomib-pr-171.html and HCoV-SARS) and human bocavirus (HBoV). Detection of these viruses has been made possible by molecular diagnostics [5-7]. The reported frequency of multiple viral findings in a respiratory specimen varies with the diagnostic methods used and between different studies, with reported frequencies between 2% and 27% [8-15]. Detection of multiple viruses in the respiratory tract may be explained by simultaneous or overlapping sequential infections. It has been discussed whether quantification of the viral load in respiratory specimens could be used to distinguish between simultaneous and overlapping infection. The amount of virus could be measured semi-quantitatively, expressed as the cycle threshold value. In our previous study [16], we compared the cycle threshold values for different viruses in specimens containing multiple and single findings. For bocavirus, the viral load was higher in single than in double infections. No difference was seen for the other compared viruses (influenza A-virus, picornavirus, coronavirus, adenovirus or RSV). Discrimination between single and coinfections was not possible using cycle threshold values.