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At a depth of 0��3�C0��6?m below ground surface, the soil was characterized as a sandy loam soil, with a texture of 71��2% sand, 16��0% silt, and 12��8% clay, pH?=?7��6, 0��36?mmhos?cm?1 of conductivity, http://www.selleckchem.com/products/Bortezomib.html and 1��7% organic matter (Harris Laboratory, Lincoln, NE, USA). The groundwater table was http://www.selleckchem.com/products/gsk1120212-jtp-74057.html the ground surface around the OWTS (Fig.?S1A). Monitoring of wastewater from the d-box, groundwater from the 11 monitoring wells, and surface water from five nearby property line ditches and the adjacent coastal receiving waters for environmental parameters (pH, water temperature, dissolved oxygen, specific conductance and salinity), FIB (total coliforms, E.?coli and enterococci) and the coliphage MS2 tracer was conducted from March through December 2008 (see Tracer tests and sample collection). In August 2009, the failing OWTS was repaired (see Data?S1). In the repaired system, septic-tank effluent from a pump tank was pumped through a pressure manifold system to drainlines located in a mounded system above a field of new, clean sand. Five monitoring wells were installed around the gravel bed (Fig.?S1B). Monitoring of wastewater from the pump tank, groundwater from the five http://www.selleck.cn/products/blu9931.html monitoring wells, and surface water from six nearby ditches and the adjacent coastal receiving waters for environmental parameters (pH, water temperature, dissolved oxygen, specific conductance, and salinity), FIB (total coliforms, E.?coli, and enterococci), and the coliphage MS2 tracer was conducted from September through December 2009 (see Tracer tests and sample collection). A viral tracer study using the male-specific coliphage MS2 was conducted in August 2008 (prerepair) and November 2009 (postrepair). A stock solution of MS2 (American Type Culture Collection, ATCC, number 15597-B1) was prepared by inoculating the ampicillin/streptomycin-resistant E.?coli host (E.?coli Famp, ATCC no. 700891) according to ATCC protocol. The final MS2 concentration in the stock solution was determined using the double agar layer procedure (USEPA 2000). MS2 was added to the d-box of the prerepaired system (2��75 �� 1010 plaque-forming units, PFU, solution) and to the pressure manifold pipe of the postrepaired system (1��36 �� 1014 PFU solution).