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Comparisons between the young and old prostates using two-way anova showed that there were no significant differences between the young and old tissue in the response to 18�� GA (Figure?4B). However, the membrane potential was more depolarized in the cells from old prostates (anova, P http://www.selleckchem.com/products/BIBW2992.html from young guinea pigs and five similar preparations from old guinea pigs [Figure?5A(a) and B(a)]. Parameters measured including basal tension, amplitude of contraction, slope and duration were not significantly affected on the addition of the drug compared to the control values for each age group (anova, P > 0.05). However, the frequency of spontaneous contractions in the prostate tissue from old guinea pigs was significantly reduced from control values; 4.3 �� 0.6?min?1 to 1.8 �� 0.4?min?1 (anova, http://www.selleckchem.com/products/BKM-120.html P http://en.wikipedia.org/wiki/SWAP70 P