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17 Cortical bone volume was identified automatically as the volume containing 5 consecutive voxels filled with bone tissue. The cortical thickness was calculated as cortical volume/outer bone surface.18 Cortical vBMD of the distal and proximal 20 slices in each scan were also analyzed and compared in 24 randomly selected children to demonstrate its change within a short range along the metaphysis. Trabecular bone volume fraction (BV/TV) was calculated as trabecular vBMD/1.2g HA/cm3, assuming vBMD of any voxel filled with mineralized tissue was 1.2?g HA/cm3. Trabecular number (Tb.N) was defined as the inverse of the mean spacing of between trabeculae identified by a mid-axis transformation method which identifies the three-dimensional ridges (centre points of the trabeculae).19 The trabecular thickness (Tb.Th) and http://www.selleckchem.com/products/ly2157299.html trabecular separation (Tb.Sp) were obtained using standard methods in histomorphometry: TbTh?=?(BV/TV)/TbN and TbSp?=?(1 �C BV/TV)/TbN. The coefficient of variation of repeated measurements with repositioning, which was done only in radius, is? http://www.selleck.cn/products/s-gsk1349572.html Data were presented as mean?��?SD unless otherwise stated. A p-value? http://www.selleckchem.com/products/z-vad-fmk.html I (p?