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? 2010 Wiley-Liss, Inc. ""Inflammatory bowel disease, including ulcerative colitis, is characterized by persistent or recurrent inflammation and can progress to colon cancer. Colitis is difficult to detect and monitor noninvasively. The goal of this work was to develop a preclinical imaging method for evaluating colitis. Herein, we report improved MRI methods for detecting and characterizing colitis noninvasively in mice, using high-resolution in vivo MR images and dynamic contrast-enhanced MRI studies, which were confirmed by histologic studies in a murine model of colitis. C57Bl6/J male mice were treated with 2.5% dextran sulfate sodium http://www.selleckchem.com/products/Bortezomib.html in their drinking water for 5 days to induce colitis. MR images were acquired using a 9.4-T Bruker scanner from 5�C25 days following dextran sulfate sodium treatment. In http://www.selleckchem.com/products/gsk1120212-jtp-74057.html dynamic contrast-enhanced MRI studies, Gd uptake (Ktrans) and its distribution (ve) were measured in muscle and normal and inflamed colons after administering Gd-diethyltriaminepentaacetic acid (Gd-DTPA). T2-weighted MR images distinguished normal colon from diffusely thickened colonic wall occurring in colitis (P http://www.selleck.cn/products/bgj398-nvp-bgj398.html 63:922�C929, 2010. ? 2010 Wiley-Liss, Inc. ""Hexafluorobenzene (HFB) and perfluoro-15-crown-5-ether (15C5) were compared as fluorine reporter probes of tissue oxygenation using 19F MRI for dynamic assessment of muscle oxygenation, with special focus on muscle tissue toxicity of the probes, and consecutive alteration of animal behavior. The latter were also compared in terms of sensitivity to changes in oxygenation as well as of signal-to-noise ratio for accurate pO2 measurements. For that purpose, mouse muscles were imaged at 11.7 T, at 2- and 36-h after intramuscular injection of HFB or 15C5. Histological analysis of the muscle tissue revealed a lack of toxicity for 15C5 from 2 up to 36-h postinjection, whereas HFB induced tissue necrosis, blood clots and thrombosis as soon as 24-h postinjection. This muscle toxicity led to a limitation in mice mobility 24-h after injection of HFB as evidenced by behavioral testing (open-field, grip strength, and catwalk tests), which was not the case after 15C5 intramuscular injection. Finally, pO2 measurements assessed 2-h postinjection showed consistent values with both probes, evidencing cross-validation of the 19F MRI oximetry technique for acute measurements. However, the measurement at 36-h was hampered for HFB, which showed significant lower values of muscle pO2, whereas 15C5 was able to reliably assess muscle pO2 at 36-h postinjection. Magn Reson Med, 2013.