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?coli and other enterobacteria, also act upon gyrase enzymes (Zamble et?al. 2001); salicylate, an inducer of the marRAB regulon, hence diminishing quinolone susceptibility, can also be synthesized by bacteria (Kerbarh et?al. 2005). The role of these naturally occurring compounds in selecting FQ resistance and the clinical impact of this low-level resistance phenotype should be analysed further. We are grateful to Eva Salinas, Leticia Mart��nez and Raul Belmont for their technical assistance. ""In Table?2 of the paper by Robertson et?al. (2009), the sequence of the 5�� 3�� 16SG forward primer should read ��GGATGAGGCATGCAAGTC�� as opposed to the current ��TGATGAGGCATGCAAGTC��. For clarity, the corrected table is reproduced below: The authors apologize for any confusion http://www.selleckchem.com/products/LY294002.html this may have caused. ""Aims:? To elucidate the cause of high variations and inconsistencies in bacterial CFU observed within and between different experiments while assessing viable bacterial counts through spread plating (SP). Methods and Results:? Following the inconsistent results, CFU estimations were undertaken through conventional SP using the spreader, or a modified approach that did not use spreader employing four organisms. The latter approach involving spotting-and-tilt-spreading http://www.selleck.cn/products/wortmannin.html of inoculum on agar surface [spotting spreading (SS)] yielded higher CFU by 11�C120% over the weighted average depending on the organism and diluent. The adverse effect owing to the spreader was http://www.selleckchem.com/products/CHIR-99021.html the most obvious in Escherichia coli followed by Staphylococcus epidermidis, Enterobacter cloacae and Bacillus pumilus. Plate attributes that determined the surface moisture levels of agar medium and the spreading practice adopted by the personnel formed two other major influencing factors. Plating for shorter periods (
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