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In order to obtain embryos and fetuses at various stages of development, caesarean sections were performed on females at the time of slaughter, in accordance with the provisions of Spanish Royal Decree 54/1995, on the protection of animals at slaughter or sacrifice. Gestational age was estimated following Evans and Sack (1973) and Sivachelvan et al., (1996), as well as in the light of age classifications previously reported for sheep (Franco et al., http://en.wikipedia.org/wiki/NK_cells 1992) and deer (Franco et al., 2004a, b). Once the rumen had been separated, small pieces of tissue were dissected from the medial region of the dorsal sac and from the cranial sac. Tissues for histological examination were fixed in 4% http://www.selleckchem.com/products/Gefitinib.html buffered formaldehyde for 24 hr, routinely processed and embedded in paraffin. Sections 5 ��m thick were stained with Hematoxylin-Eosin (H&E), Masson's Trichrome and Gomori's reticulin. Specimens for morphometric analysis were embedded in paraffin, stained with H&E, and viewed through a microscope (NIKON Eclipse 80i) equipped with a digital video camera (NIKON DXMI200F). The computerized image was analyzed using the Nis-Element 2.30 software package. The variables studied were height of various tissue strata (epithelium, lamina propria and submucosa, tunica muscularis, and serosa) and total wall thickness. Ten specimens were selected for each group for histology and morphometric study. In Group I of these 10 individuals, 2 belonging to the age of 13 days, 3 in 35 days, and 5 for 38 days; in Group II, 3 fetuses of 39 days, 3 of 46 days and 4 of 50 days were selected; in Group III, 2 fetuses of 53 days, 3 of 62 days, 3 of 70 days, and 2 of http://www.selleckchem.com/products/DAPT-GSI-IX.html 74 days were chosen; in Group IV, were selected 3 fetuses of 76 days, 3 of 84 days, 2 of 95 days, and 2 of 112 days were selected; in Group V, 2 fetuses of 113 days, 3 of 125 days, 3 of 138 days and 2 fetuses of 150 days were chosen. One hundred measurements were made in each tissue layer (epithelium, lamina propria + submucosa, tunica muscularis, serosa and total thickness of the rumen wall) of each of the selected individuals from each group. The results are shown as mean �� SE. Data was subjected to analysis of variance (ANOVA). Wherever ANOVA revealed significant differences, a post-hoc (Turkey) analysis was carried out to test for significant differences between tissue strata and groups. A value of P