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0 or 0.5?mm slice collimation (Aquilion-16, Aquilion-64, AquilionONE; all from Toshiba Medical Systems, Otawara, Japan). The examination protocol included the application of 120?ml of an iodinated contrast agent (Ultravist, 370?mg iodine/ml, Bayer HealthCare http://www.selleck.cn/products/MK-1775.html Pharmaceuticals, Berlin, Germany; Xenetix, 350?mg iodine/ml, Guerbet, Roissy, France) at a flow of 2.5�C3.5?ml/s. Examinations were performed in arterial and venous phases with a delay after the start of contrast injection of 30s and 60s, respectively. Images were reconstructed for thin slice data sets at 0.5�C1.0?mm for post-processing (Fig.?1). For assessment of renal cortex volume CT images were copied to a post-processing workstation (Vitrea core, Vital images, Minnetonka, MN, USA). As a first step, images were reconstructed in axial, sagittal and coronal views using the thin slice data sets. Then the software measured the cortex volume of the kidney by automatical volume rendering function based on density differences of the cortex compared to the surrounding tissues. Adjustments of the segmentations were done manually using the multiplanar reconstructions. Pre-donation split renal function, http://www.selleckchem.com/PD-1-PD-L1.html e.g., the side-specific GFR of each kidney, was calculated via multiplying the percentage of either CT-measured split cortex volume (Vol%) or MAG3- measured split renal function (MAG3%) by total GFR. Pre-donation split cortex volume was calculated via multiplying the percentage of CT-measured split cortex volume (Vol%) or MAG3-measured split renal function (MAG3%) by total cortex volume. Statistical analysis was performed using spss statistical software (version 19.0; IBM Corp., Armonk, NY, USA) and graphpad prism software (version http://www.selleckchem.com/products/Adriamycin.html 5.0; graphpad Software, Inc., LA Jolla, CA, USA). Data are shown as mean?��?SD. Cortex volume and all GFR values in donors pre-transplant and recipients post-transplant were adjusted to body surface area (BSA) using the Du Bois formula [18]. Relationship between two parameters was analyzed by correlation analysis (Pearson) and linear regression analysis. Bland-Altman test was employed to assess reliability and agreement between the different methods of GFR assessment. A probability of