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3). For example, the fast-developing isolates A66, A75 and A98 do not cluster together in a common clade, but are distributed across the phylogeny, as are the two slowest-developing strains, GJV1 and A94. Ancestral character states for the rate of development are difficult to infer for several nodes. A subset of strains representing slow (GJV1, A9, A94), moderate (DK836, http://www.selleck.cn/products/carfilzomib-pr-171.html Mxx144, A41, A85) and fast (A66, A75, A98) rates of developmental morphogenesis was also examined for variation in the levels and patterns of spore production at six time points after the onset of starvation ranging from 24 to 168?h. Spore production varied significantly across strains (one-way anova, F=26.75, df=9, P http://www.selleckchem.com/products/BIBF1120.html order of time to initial developmental opacity and the ranked maximum number of spores produced (Spearman's rank test, rS=0.89, n=10). In other words, http://www.selleckchem.com/products/Metformin-hydrochloride(Glucophage).html early aggregating strains tended to rank higher in maximum spore production than slow-developing strains, indicating the absence of a trade-off between the rate and the productivity of development. The number of spores produced that remain viable after 2?h of heating at 50, 55, 60 and 65?��C was determined for strains GJV1, DK836, Mxx144, A66, A75 and A98 (Fig. 5). Overall, the number of surviving spores was predicted by temperature treatment, strain and temperature by strain interaction (GLM, F=78.94, df=2, P