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About 100?CFU?25?g?1 of L.?monocytogenes was artificially introduced into food samples. SEL and ?FB were used as enrichment broths. Actual cell numbers inoculated into the food samples were 229?CFU?25?g?1, and bacterial cell counts were reached to about 4?��?107 and 3?��?107?CFU?ml?1, respectively, after 18?h of enrichment in all meat samples. Signals from inoculated beef, chicken and turkey that were enriched with SEL showed average values of 5200, 6800 and 6000?pA, respectively, while the signals were 2600, 5000 and 7600?pA in ?FB, respectively (Fig.?4). When these signal values were compared with values for uninoculated samples, only the SEL-enriched samples showed statistically http://www.selleckchem.com/products/gsk1120212-jtp-74057.html significant difference (P? http://www.selleck.cn/products/blu9931.html successfully be used for detection of L.?monocytogenes from contaminated ready-to-eat meat samples. Furthermore, PCR analysis confirmed the presence of L.?monocytogenes in each meat sample (Fig.?4c) corroborating the positive fibre-optic signal. Although the lower limit of the sensor was 103?CFU?ml?1 of bacterial cells, the fibre-optic biosensor could successfully detect lower inoculum size of 102?CFU?25?g?1 (4?CFU?g?1) of sample after a period of enrichment. This number corresponds to below 1?CFU?ml?1 because 25?g meat was enriched in 225?ml of broth. In this study, our goal was to develop a Listeria monocytogenes-specific fibre-optic sensor employing antibody�Captamer for rapid detection of this organism from food. Aptamer http://www.selleckchem.com/products/Bortezomib.html was generated against InlA, a virulence factor present only on pathogenic Listeria (Jacquet et?al. 2004; Bierne et?al. 2007), labelled with Alexa Fluor 647, and used as the reporter molecule in an antibody�Canalyte�Captamer sandwich configuration. The specific binding of aptamer to InlA on the surface of L.?monocytogenes cells was confirmed using fluorescent microscopy and immunoblot assay. Most of the L.?monocytogenes express full-length functional InlA on the surface, but in some cases, the truncated form of the InlA, which is generally nonfunctional, is also made.