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(iii) Isolates from southwest-1 and -2 clustered together, indicating a closer genetic relationship between them, in line with their geographic origin. Overall, none of the isolates appeared to be identical, although two isolates (26 and 29) had about 96% similarity in their peak profiles (Fig.?3). Principal coordinate analysis (PCO) also revealed the intraspecies subdivision among the C.?sublineolum isolates from the different parts of Ethiopia (Fig.?4). The first three principal coordinates (PCO1-3) accounted for 34��5%, 29��2% and 15��2% of the total variation, respectively. Results of the PCO analysis were more or less in line with those of the upgma clustering, but the http://www.selleck.cn/products/AZD0530.html PCO analysis clearly separated the isolates from the south and east from the others, whilst reducing the overall number of groups. Isolates from the south spread over quadrants I and II of the PCO graph, whilst the isolates representing the eastern region grouped together in quadrant II, with one isolate slightly separated from the others. Isolates from the two southwest sites and the north grouped together, scattered over the third and fourth quadrants. The analysis of molecular variance (amova) indicated a high level of genetic variation both between isolates from different sampling sites (42%) and within sampling sites (58%) (Table?3). Results from this analysis revealed http://www.selleckchem.com/products/forskolin.html a significantly high (P? http://www.selleckchem.com/products/cx-5461.html differentiation and limited gene flow (Nm?=?0��343). Pairwise genetic differences among the different groups were also high, further indicating subdivision of C.?sublineolum according to region. Generally, the group of isolates from South Ethiopia, which had an average pairwise difference of 0��48 from groups of isolates from other regions, was found to be the most genetically divergent group, even though it is not geographically the most isolated region (Table?4). The highest pairwise difference (0��51) was recorded between isolates from the south and the east, indicating that they were the most separated in terms of genetic distance. In contrast, the two groups of isolates from southwest Ethiopia (southwest-1 and -2) had the lowest pairwise difference (0��11), making them the genetically most related groups in this study. The findings reported here are from the first comprehensive study to characterize C.?sublineolum isolates from different sorghum-growing regions of Ethiopia. Isolates were collected from five different sorghum-producing areas in four geographic locations and characterized using phenotypic characters, i.e. growth rate, colony colour and margin, as well as AFLP fingerprinting. In the current study, phenotypic characterizations of C.?sublineolum isolates representing different regions revealed considerable variations in growth rate, but very little difference in colony morphology (pigmentation and margin).