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2010) to calculate NRI. To determine how the measured response variables (e.g. species diversity, mean Bray�CCurtis dissimilarity, CWM traits, difference in FDis, NRI, CVFDis) changed along the environmental gradient, we focus on the direction and strength of correlations and comparisons across correlations, rather than statistical significance due to the chance effects, http://www.selleckchem.com/products/Rapamycin.html using a general linear model with PC1 (or mean PC1 in the case of CVFDis) as a continuous variable to describe the environmental gradient. For each response measure, we tested for both linear and nonlinear relationships. We selected the best fit using Akaike Information Criteria (Crawley 2007). Above-ground biomass in plots ranged from 36.5 to 758.0?g?m?2 and was positively correlated with soil depth, soil moisture and inorganic nitrogen availability. This set of environmental factors was described by the first principal component axis (PC1), which accounted for 46% of the variation in the environmental data. Differences in nitrogen mineralization among plots were described by principal component 2, which accounted for an additional 19% of the variation among plots (see Appendix?S1 in Supporting Information). In the subsequent analyses, we use the first axis scores as the descriptor http://www.selleckchem.com/products/Everolimus(RAD001).html of the environmental gradient, running from stressful areas (characterized by low above-ground production, shallow and dry soils, low inorganic N) to more-resource-rich areas (higher biomass production, soil moisture and inorganic N). Greater exposure to wind and cold also occur at the stressful end of this gradient (Walker et?al. 1993; Bowman & Fisk 2001; Williams, Helmig & Blanken 2009). There were 75 species in the study area species pool. Community species richness varied from 11 to 29 species?m?2. Species http://www.selleck.cn/products/PD-98059.html richness and Simpson��s diversity index decreased linearly along the stress gradient (F1,78?=?38.92, r2?=?0.33, P?