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A typical trace is shown in Figure?2B and other data are given in Table?2. Contractile activity.? Two concentrations of 18�� GA (10??M and 40??M) were used to study its effects on spontaneous contractions, in prostate tissues from young and old guinea pigs. At the lower concentration (10??M), 18�� GA reduced amplitudes of contraction in tissues from both age groups, relative to the control values (0.38 �� 0.2?N��g?1 to 0.16 �� 0.02?N��g?1 in young, and 0.51 �� 0.2?N��g?1 to 0.10 �� 0.02?N��g?1 in old), although this treatment did not have an age-related effect (anova, P > 0.05). In contrast, the frequency of contractions was significantly reduced in old prostate tissue from 4.6 �� 0.6?min?1 to 2.0 �� 0.7?min?1 (anova, P http://www.selleckchem.com/products/BIBW2992.html concentration of 18�� GA (40??M), spontaneous contractile activity was abolished in four of five preparations across both age groups (anova, P 0.05). The responses to phenylephrine were again unaffected by 18�� GA at this higher concentration, in tissues from young and old guinea pigs http://en.wikipedia.org/wiki/SWAP70 [Figure?3A(b) and B(b)]. Electrical activity.? 18�� GA (40??M) was added to cells, exhibiting slow wave activity, taken from prostate glands of young guinea pigs. Under the control conditions, the amplitude of depolarizing transient was 11.3 �� 1.2?mV and the number of spike potentials was 2.3 �� 0.2 (n= 9). Other variables measured in these cells under control conditions are summarized in Table?3. The addition of 18�� GA caused a time-dependent decrease http://www.selleckchem.com/products/BKM-120.html in slow wave activity, such that after 2?min of exposure to the drug, activity was abolished (Figure?4A). The membrane potential was significantly depolarized from that under control conditions (Table?3; Student's paired t-test, P