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2008). ECP-100 turned out to be an effective solution after application on contaminated cantaloupe slices (application with a pipette) and fresh-cut lettuce (treatment by spraying) stored at refrigeration temperature (4��C) (Sharma et?al. 2009). In experiments by Viazis et?al. (2011), artificially contaminated organic baby spinach and baby romaine lettuce leaves purchased at a local market in the USA were treated with a mixture of eight lytic E.?coli O157:H7-specific phages (107?PFU?ml?1) at different multiplicity of infection (MOI) levels (1, 10 and 100) and under various conditions http://www.selleck.cn/products/nlg919.html (at 8, 23 and 37��C for 10?min, 1?h and 24?h). Treatment significantly (P? http://www.selleckchem.com/products/pexidartinib-plx3397.html Several articles have described attempts to control E.?coli O157:H7 with the ready-to-use commercial preparation EcoShield, developed by Intralytix, Inc. Boyacioglu et?al. (2013) and Ferguson et?al. (2013) investigated application of this preparation on fresh-cut leafy greens. http://www.selleckchem.com/products/bmn-673.html EcoShield consists of three different E.?coli O157:H7-specific phages and recently has been approved by the FDA and USDA Food Safety and Inspection Service for use on meats and on food contact surfaces. In one of the performed experiments (Ferguson et?al. 2013), leaves of iceberg lettuce obtained from a grocery store were immersed in phage solution (108�C109?PFU?ml?1 for 30?s or 2?min). After drying, 50?��l of bacterial suspension (105?CFU?ml?1) was applied to the surface of leaves. Pieces of lettuce were stored for up to 7?days at 4��C, which is a typical storage period at grocery stores. Boyacioglu et?al. (2013) treated purchased fresh spinach and romaine lettuce first with an E.?coli O157:H7 dilution (107?CFU?ml?1), and after 20?min, an EcoShield suspension (107?PFU?ml?1) was sprayed onto cut leaves. The leaf pieces were stored for up to 7?days at 4 and 10��C. In both cases, phage application significantly (P?