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Testing was conducted in a quiet room, in a three-sided black-curtained enclosure. Participants were seated approximately 45cm from the monitor; ambient light levels were reduced to diminish distraction. https://en.wikipedia.org/wiki/Pentamorphone Verbal instructions, limited to ��Look at the TV��, were used at the beginning of the session. To minimize body and head movement, all participants with Rett syndrome (and all typically developing participants http://www.selleckchem.com/products/LBH-589.html Attention was assessed by measures of total looking time, number of fixations, and the average duration of fixations; recognition memory was assessed by novelty scores. For each measure, two composites were formed: one by averaging the five face problems, the other by averaging the four pattern problems. Problems having less than 1?second of looking on familiarization or test were excluded when forming composites (23% for Rett group, 7% for comparison group). Face composites were missing for two patients with Rett syndrome and pattern composites for one patient with Rett syndrome. The distributions of all composites were examined for outliers; because of positive skew, familiarization variables were http://www.selleckchem.com/products/Adriamycin.html square-root transformed. The effects of Rett syndrome were tested using t-tests for age, familiarization variables, and novelty scores; single-group t-tests were used to compare novelty scores for each group against chance performance (50%). Composites involving the three AOIs (eyes, nose, and mouth) were analyzed using repeated-measures multivariate analyses of variance (MANOVAs), with group as a between-participant factor; significant univariate results were explored with Scheff�� post-hoc tests. Cases were dropped from a MANOVA if data on any of the three AOIs were missing. Relations among clinical characteristics and VPC variables were assessed with point-biserial or Pearson's correlations, as appropriate. SPSS (version 19; SPSS Inc., Chicago, IL, USA) was used for all analyses; a two-tailed 0.05 criterion for significance was applied throughout. Both groups showed interest in the faces, spending 61% to 70% of the 10-second familiarization period looking at them (Table?2 and Fig.?2). Although the duration of looking to faces did not differ across groups (t53=1.45, p=0.15), the Rett group showed a less mature pattern of looking, having fewer and longer fixations (t53=2.75, p=0.008, and t53=1.96, p=0.05 respectively).