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The ratio of the observed to expected recovery for mannitol ranged between 84% and 100% and for lactulose ranged between 90% and 113% across all dilutions, suggesting http://www.selleckchem.com/products/Roscovitine.html there was sufficient linearity of the assay. The accuracy of the assay lay between 97% and 116% for mannitol and 93% and 113% for lactulose, respectively. Based on the recoveries of the sugars, the LOD for lactulose and mannitol in the urine samples was 15?��g?mL?1. The precision and reproducibility of the assay for intra-assay and inter-assay variability (CV%) for lactulose and mannitol was between 2.0% and 5.1%, and 2.5% and 4.4%, respectively. There was no significant correlation between either the total quantity of mannitol or the total quantity of lactulose excreted and the total volume of urine produced. The LMR of urines increased in a curvilinear manner over time with incremental half hourly increase tending to decrease after 5?h. The overall LMRs of the urines collected during the first 3?h (mean: 0.04?��?0.03) were significantly lower on repeated measures anova than those collected over the second 3?h (mean: 0.1?��?0.06) (df 1,19; F?=?48.74; P? http://www.selleckchem.com/products/Neratinib(HKI-272).html samples collected over any of the periods of collection (Table?2). Temporal variation in excretion of mannitol? There was a (predictable) significant (df 1,19; F?=?141.39; P? https://en.wikipedia.org/wiki/Quinapyramine over the succeeding 3?h of collection. The excretion pattern of mannitol did not differ between smokers and non-smokers (Table?2). Temporal variation in excretion of lactulose? There was an overall (predictable) significant increase (df 1,19; F?=?202.55; P?