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The primary antibody against VEGF detected two (VEGF121 and VEGF165) of the four VEGF isoforms (Hyodo et?al. 1998). The standard curve http://www.selleckchem.com/products/MK-1775.html was plotted from the measurements taken with the standard solution (20.6�C1000?pg/ml) and the VEGF concentration in the sample was determined. The assay was performed according to the manufacturer��s instructions. The limit of the detectable VEGF concentration was 20.6?pg/ml. The size of the mCNV was measured on fluorescein angiography before treatment. The fluorescein angiography images were digitalized using ImageNet? (Topcon, Tokyo, Japan), and both the mCNV and the disc size were measured using the ImageNet? software. The mCNV area was divided by the disc area and the mCNV size was expressed in disc areas. The axial length was measured using an IOL Master? (Carl Zeiss Meditec, Jena, Germany) in the patients with mCNV. The data were analysed using SIGMASTAT software (version 3.1; Systat Software Inc., Richmond, California, USA) and expressed as the median value. The differences between the VEGF concentrations in the aqueous humour of patients with mCNV and the control patients were compared using the Mann�CWhitney rank sum test. The Spearman rank-order correlation http://www.selleckchem.com/products/AZD6244.html coefficient test was used to examine the correlation between the VEGF concentrations in the aqueous humour and the size of the CNV or the axial length. A p-value http://www.selleck.cn/products/azd4547.html and cataract. The VEGF concentrations in the aqueous humour in eyes with mCNV ranged from
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