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The functional categories, the most affected by the introgressed fragment, were the hormonal and stress responses (the latter being mostly up-regulated in C9d) as well as, to a smaller extent, the protein metabolism and transcription categories (the latter being mostly down-regulated in C9d). The effect of FL on tomato fruit transcriptome in each genotype was analysed by comparing gene expressions under HL and LL in M and in C9d (Fig.?3a). In total, 103 genes out of 11?862 (0.9 %) were differentially expressed in M versus 147 in C9d (1.2 %) (Fig.?3c). Among them, 56 differentially expressed genes were common to C9d and M with similar responses, whatever the genotype, except for one unigene showing an opposite response depending on the genotype. http://www.selleck.cn/products/PD-98059.html Figure?4b displays the distribution of the differentially expressed unigenes into functional categories (Supporting Information Tables S4 and S5). According to the MapMan classification, all biological processes were affected by FL but to a variable extent. As expected from the modification of the source/sink relationships under LL, the expression of genes implicated in primary and secondary metabolism was indeed affected. Accordingly, LL fruits also displayed changes in cell wall-related genes (mostly up-regulated) and in electron transport (mostly down-regulated). A large proportion of http://www.selleckchem.com/products/Rapamycin.html the differentially expressed genes, mostly down-regulated, have no function attributed. However, the most striking differences between HL and LL �C in terms of expression http://www.selleckchem.com/products/Everolimus(RAD001).html level �C were observed for genes involved in signalling, in regulation of gene expression (hormonal responses, transcription) and in protein metabolism (protein synthesis, post-translational modifications and degradation). Details of several categories showing remarkable changes are displayed in Table?1. In primary metabolism, the enzyme exhibiting the highest changes under LL conditions in both M and C9d genotypes (log2-fold-change?=???1.6 in M, ??1.5 in C9d) was the fructose-2,6-bisphosphatase (F26BPase). Several differentially expressed genes with putative function in signalling exhibited high fold-changes under LL conditions in both M and C9d (1.4?