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At harvest total root and tuber nematode densities were calculated for all experiments together with the nematode reproduction factor from the screenhouse pots. Data were subjected to analysis of variance according to the general http://www.selleckchem.com/products/cx-5461.html linear model procedure using the SAS (SAS Institute Inc., 1997) statistical package. Data of nematode density and rot were (log10 (X?+?1)) transformed prior to analysis to attain normal distribution among treatments. Treatment means were compared using the Least Significant Difference test (LSD) at P?��?0��05. In the sterilized soil in the screenhouse, inoculation of S.?bradys caused severe damage to potatoes (Tables?1 & 2). The nematode infected both roots and tubers, resulting in substantial surface cracking of tubers, and root and tuber http://www.selleckchem.com/products/forskolin.html rot, 8?weeks after inoculation with 1000�C5000 specimens of S.?bradys, compared with the nearly healthy tubers from non-inoculated plants (Fig.?1). Symptoms of the nematode infection also included scaly appearance, deep tissue cracks and distortions, and darkened surface patches, in most cases associated with sub-surface rot. Root fresh weight was affected (P? http://www.selleck.cn/products/AZD0530.html to 1��0 nematodes cm?3 of soil) and declined in pots inoculated with the highest inoculum level (5000 S.?bradys per pot, equivalent to 2��0 nematodes cm?3 of soil) (Table?3). In the field soil, no S.?bradys was recovered before planting, while trace numbers of nematodes within the genera Meloidogyne and Pratylenchus were detected, and Helicotylenchus spp. were present in low densities (
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