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single), pulmonary artery systolic pressure (PASP) at the time of transplantation http://www.selleckchem.com/products/nutlin-3a.html and GERD were evaluated as possible confounders based on a hypothesized association with sRAGE and BOS. We generated a multivariable model including grade 3 PGD at 72 h, ACR, peak FEV1, diagnosis and transplant type, regardless of whether they met the statistical definition for confounding, as these variables might be important in the relationship between sRAGE and BOS. Plasma samples were obtained in citrated tubes 6 and 24 h after reperfusion of the lung allograft(s). Samples were centrifuged within 60 minutes of collection and stored at -80��C. Plasma levels of sRAGE were measured by sandwich ELISA (R&D, Minneapolis, MN, USA). The intra-assay coefficient of variation was 6%. We created a standardized sRAGE variable by dividing each plasma sRAGE measurement by the standard deviation of sRAGE from the cohort and used this in the analyses. Descriptive statistics were generated using chi square tests for categorical variables and two-tailed Student t-tests for continuous variables. Analysis of the relationship between sRAGE levels and time to BOS was performed using Cox regression models. In secondary analysis, we accounted for death http://www.selleckchem.com/products/AC-220.html as a competing risk to ensure there was no change in the association between sRAGE and BOS when considering subjects who died before development of BOS [20]. We evaluated sRAGE both as a continuous variable and as a categorical variable, using quartiles. We used a bivariate confounder selection strategy, and confounding was defined as a greater than 20% change in hazards ratio between sRAGE and BOS upon addition of a covariate to the model [21]. Longitudinal analyses using generalized estimating equations were performed to explore a relationship in trend of FEV1 over time with quartiles of sRAGE. In sensitivity analyses, we analyzed the multivariable model without peak FEV1, as this variable is encompassed in the definition of BOS http://www.selleck.cn/products/Romidepsin-FK228.html [1]. For all of the statistical analyses, a p
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