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2). For statistical calculations, the SAS program (Version 9.1, SAS, Cary/NC, USA) was used. The amount of apoptotic cells in LE and LP was compared by means of the Student's t-test independent of the cycle stage. Further comparisons were performed between oviductal regions (UTJ, isthmus) and staining techniques. The occurrence of cyclic changes in the number of apoptotic cells was detected by one-factorial analysis of variance and differences between http://www.selleckchem.com/screening/epigenetics-compound-library.html specific stages of the estrous cycle were analyzed using the t-test for independent samples P http://www.selleckchem.com/products/Dasatinib.html https://en.wikipedia.org/wiki/Evodiamine (anestrus, early and late follicular phase, endometrial reparation). Regardless of the estrous cycle stage and the cell layer (LE or LP), significantly more apoptotic cells were detected in the UTJ (7.9 �� 6.5) than in the isthmus (5.4 �� 4.5) by using the TUNEL assay but no such difference was found verifying caspase-3 activity (Table 1). For presenting cyclic influences on apoptosis in the canine UTJ and caudal isthmus only the results obtained for the LE are considered as the proportion of cells showing signs of apoptosis was on a constantly low level both in the LE and LP Regarding the two techniques for verification of apoptotic cells in the LE of the UTJ and isthmus, no statistical difference was found throughout the estrous cycle except in one stage each: in the UTJ in anestrus and in the isthmus at 4 days after ovulation (P
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