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Mortality was ?16% higher for eggs in the desiccated and submerged treatments than for eggs in the hydrated treatment (Fig.?2; GLM, F2,48?=?8��79, P?=?0��0006). Mean hatching time and the synchrony of hatching varied for egg masses in the three environments (Fig.?2; hatching timing: LM, F2,48?=?72��82, P? http://www.selleck.cn/products/bmn-673.html onset to completion of hatching (Fig.?2). Hydrated eggs hatched later (85��9?��?1��4?h post-oviposition) and over a greater period of time (32��4?��?2��0?h). Submerged eggs did not hatch until 97��0?��?0��9?h post-oviposition and took 52��6?��?2��3?h to hatch (Fig.?2). Hydrated eggs began hatching 69��5?��?1��9?h after oviposition, significantly earlier than desiccated eggs (75��8?��?0��6?h; Fig.?2; LM, F2,48?=?3��73, P?=?0��031; Tukey��s post-hoc comparison, P?=?0��027). Neither group differed from submerged eggs, which began hatching at 71��6?��?2��0?h (Fig.?2). Despite hatching later, hatchlings from submerged eggs were less developed than hatchlings from either terrestrial environment [Fig.?3; Gosner stage: hydrated?=?23��1?��?0��2, http://www.selleckchem.com/products/Everolimus(RAD001).html desiccated?=?22��9?��?0��2, submerged?=?21��5?��?0��3 (Gosner 1960); LM, F2,48?=?16��24, P? http://www.selleckchem.com/products/PD-0332991.html smaller than those from hydrated eggs, but neither was different in size from hatchlings from desiccated eggs (Fig.?3; TTL: hydrated?=?5��56?��?0��06?mm, desiccated?=?5��41?��?0��06?mm, submerged?=?5��26?��?0��09?mm; LM, F2,48?=?4��50, P?=0��016; Tukey��s comparison, submerged �C hydrated, P?=0��011). Hatchling pigmentation varied by both egg treatment and stage at hatching, and there was no significant interaction between the two (Fig.?3d; LM, egg treatment: F2,45?=?3��79, P?=?0��029; stage: F1,45?=?19��27, P?=?0��00001; egg treatment?��?stage, F2,45?=?2��58, P?=?0��087). Pigmentation area increased with developmental stage, and was greatest in hatchlings from terrestrial egg treatments (Fig.?3d). However, because hatchlings were at different developmental stages from one another, they did not share a common size axis (i.e. the covariance of morphological measures was different between groups) and morphology could not be size corrected for comparisons of shape (Fig.?3; CPCA, P?=?0��0002). Egg environment affected survival immediately post-hatching. Hatchlings were placed in predation trials in accordance with their hatching pattern (after 15 eggs had hatched in a clutch), and as a result predation trials did not all begin simultaneously (start of predation trials, hydrated?= 76��6?��?1��8?h, desiccated?=?78��0?��?0��8?h, submerged?= 82��0?��?2��0?h; LM, F2,48?=?3��36, P?=?0��04).