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Four stands exhibited crown decline without root lesions. These stands were located in Los Alerces National Park, (42��50��75��3��S, 71��30��43��5��W), on the road to Los Alerces National Park near the Percey river (42��58��58��3��S, 71��29��37��4��W), http://www.selleck.cn/products/bgj398-nvp-bgj398.html at Estancia Santa Teresita, Corcovado, Chubut (43��32��15��S, 71��33��00��W) and Cerro Llao-Llao, Nahuel Huapi National Park (41��02��21��8��S, 71��33��01��77��W). In these cases roots were unaffected (even in trees with dead crowns) and necrotic lesions typical of P. austrocedrae were not present at the base of the trees. In addition, site conditions at these stands appeared to be less favourable for Phytophthora development (i.e. well-drained soils, steep slopes). In these stands the cause of mortality remains unknown. Nineteen inoculations of P. austrocedrae (10 of isolate CIEFAP Py190 (T1) and nine of isolate ATCC MYA4074) were made on roots, and 20 inoculations (10 of isolate CIEFAP Py190 (T1) and 10 of isolate ATCC MYA4074) were made on stems. All inoculations of P. austrocedrae on roots developed into brown, necrotic lesions in the phloem, reaching and staining the sapwood, and so did 18 out of 20 stem inoculations. There was only one inoculated tree where no lesions developed from the stem inoculations, presumably as a result of inoculum failure, but lesions developed from root inoculations of the same http://www.selleckchem.com/products/Bortezomib.html tree. Since the Levene test showed unequal variances in lesion size (P? http://www.selleckchem.com/products/gsk1120212-jtp-74057.html longer and wider (one-sided P?