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HRFO was provided from Inner Mongolia of China, CFO was achieved by using the Komet Vegetable Oil expeller, and then centrifuged (Type: Oil press CA59G; Oil temperature: http://www.selleckchem.com/products/liproxstatin-1.html https://en.wikipedia.org/wiki/Ketanserin were allowed ad libitum access to food and tap water. The chow diet was provided by the Experimental Animal Center of Disease Control and Prevention Center of Hubei Province. The high fat diet contained 82.7% chow diet, 6% butter, 10% corresponding flaxseed oil, 1% http://www.selleckchem.com/products/midostaurin-pkc412.html cholesterol, and 0.3% bile salts. All diets were prepared weekly and stored at ?80��C. The experimental period was 4 wk. Feed and water were changed every day and body weights of rats were recorded twice every week. Daily feed (g) were calculated on a per rat daily basis. At the end of experiment, rats were anesthetized by 0.2% pentobarbital sodium and then sacrificed, fasting blood was collected in 0.2% EDTA, plasma was obtained by centrifugation 1500g for 15?min at 4��C, and then stored at ?80��C. Liver from each rat was excised, washed in ice-cold saline, weighted, and stored at ?80��C for further analysis. Feces was collected from each cage for 24?h before the end of experiment, and stored at ?80��C after freeze-drying until analysis. Lipids were extracted from plasma according to Folch et al. 15, and total fatty acids were transmethylated based on the method of Agren et al. 16, FAME were then separated by Agilent 6890 GC using an HP-88 fused silica capillary column (100?��?0.25, 0.2??m) and a FID, the oven temperature began at 50��C and held for 1?min, increased to 175��C at 15��C/min, and then increased to 250��C at 1��C/min. The injector temperature and detector temperature were set at 250��C, helium was used as a carrier gas (1.5?mL/min), and the split ratio was 1:20.