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Epidermal root cells were photobleached and the early recovery of fluorescence in the ROIs was monitored for the indicated time. The line (red color) represents the fitting curve of the whole set of data. Figure S3. Kinetics of recovery http://www.selleckchem.com/products/VX-765.html after photobleaching of AtPIP-GFP constructs upon drug pretreatment. Same legend as in Figure 5b. The recoveries after photobleaching were monitored for 30?min with cells expressing the indicated AtPIP-GFP constructs. The same control curve of mock conditions is shown for the three treatments with a same construct (closed symbols). The graphs show mean values with standard errors and the numbers of ROIs analysed (n). Figure S4. Quantification of relative FM4-64 uptake in mock- and salt-treated root epidermal cells. (a) Roots were incubated in mock or 100?mm NaCl conditions for 1?h prior to addition of the endocytic tracer FM4-64 (8?��m). To follow the dye intracellular accumulation, images were acquired after 5?min. (b) A threshold was adjusted for each image. The number of pixels above the threshold value was counted using a ImageJ software, and divided by the number of pixels covering the cell interior (and excluding the PM). Error bars indicate SE. Asterisk indicates P? http://www.selleck.cn/products/azd6738.html http://www.selleckchem.com/products/pf-562271.html and values were obtained by analysing images acquired manually at the indicated time. Two types of control curves corresponding either to drug pretreatments without further salt treatment (open symbols) or to salt treatment without drug pretreatments (closed symbols) are shown. The graphs show mean values with standard errors and the numbers of ROIs analysed (n). As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. ""Large numbers of sequences are now readily available for many plant species, allowing easy identification of homologous genes. However, orthologous gene identification across multiple species is made difficult by evolutionary events such as whole-genome or segmental duplications. Several developmental atlases of gene expression have been produced in the past couple of years, and it may be possible to use these transcript abundance data to refine ortholog predictions. In this study, clusters of homologous genes between seven plant species �C Arabidopsis, soybean, Medicago truncatula, poplar, barley, maize and rice �C were identified.