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A significant correlation was observed (r?=?0��3) between chlorophyll a (plankton bloom) and an increase in the isolation percentage for non-O1�Cnon-O139 from planktons (P? http://www.selleckchem.com/products/PD-0325901.html April 2007 and 2008, with a peak during the rainy season (July�CSeptember). Clinical cholera cases correlated with rainfall (r?=?0��82, P? http://www.selleck.cn/products/Cisplatin.html for the sto/stn gene. A total of 36 antimicrobial resistance profiles were observed among environmental and clinical isolates (Table?4). Of the 147 environmental isolates, 45 were susceptible to all of the antibiotic agents tested and 102 isolates were resistant to one or more antibiotics. The percentages of resistance to the antimicrobial agents amikacin, http://www.selleckchem.com/products/3-methyladenine.html tetracycline, neomycin, norfloxacin, cefoperazone�Csulbactam, nalidixic acid, cotrimoxazole and amoxicillin were 7��45, 8��0, 9��3, 10��5, 21��7, 24��2, 28��5 and 35��4, respectively. Most of the clinical isolates were sensitive to antimicrobials, with the exception of cotrimoxazole and nalidixic acid, to which all isolates were uniformly resistant. In addition, 66��7% of isolates from 2008 were also resistant to tetracycline. A total of 251 V.?cholerae isolates were subjected to RAPD (99 clinical isolates, 147 environmental isolates and five reference strains). The RAPD pattern differentiated V.?cholerae isolates into 46 types, A-T1 (Table?5). The most common pattern was designated type A (149 isolates), followed by type D (13 isolates), type I1 (nine isolates), type H (seven isolates) and type H1 (five isolates). The largest, type A contained 149 isolates: 96 clinical isolates, 49 environmental isolates and four control strains (ElTor, MTCC, hybrid and NT5394). The environmental isolates included 19 from fixed sites, 16 from outbreak sites and 14 from large natural bodies of water. The V.?cholerae O1 Classical 569B control strain exhibited a unique pattern (type B) that was not found among the other isolates.