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These results suggest that processing of pmel17 is affected by the ADAM protease inhibitor. D.-S. Kim1, H. Li1, M. C. Balcos1, J. S. Shin1, H.-Y. Yun1, K. J. Baek1, N. S. Kwon1, K.-C. Park2 1Department of Biochemistry, Chung-Ang University College of Medicine, Seoul2Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea The purpose of this study was to investigate effects of myriocin, an inhibitor of serine palmitoyltransferase, on melanogenesis. Myriocin had no cytotoxic effect on Mel-Ab cells at concentrations http://www.selleckchem.com/products/3-methyladenine.html expression via phosphorylation of CREB, but it did not directly activate tyrosinase, a rate-limiting melanogenic enzyme. Importantly, only phospho-CREB was increased time-dependently, while Akt, GSK-3��, and p-38 were not phosphorylated http://www.selleck.cn/products/Cisplatin.html and ��-catenin level was not changed. Furthermore, we demonstrated increased melanin synthesis with myriocin on a pigmented skin equivalent model established using Cervi cornus Colla (deer antler glue). One and 5?��M of myriocin darkened the color of the skin equivalent. Moreover, the skin equivalent section was stained by Fontana-Masson to detect melanin. Melanin pigment was detected at a greater level in the myriocin-treated skin equivalent than in the untreated control. In conclusion, myriocin promoted melanogenesis through phosphorylation of CREB and increased MITF and tyrosinase levels. Furthermore, the pigment-inducing effect of myriocin was confirmed in a skin equivalent model containing Mel-Ab cells. Therefore, we suggest that myriocin could be used for the treatment of vitiligo or sunless tanning. This study was http://www.selleckchem.com/products/PD-0325901.html supported by a grant of the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea. (Grant No. A103017). D.-S. Kim1, H. Li1, J. Kim1, H.-G. Hahn2, J. Yun2, H.-S. Jeong1, H.-Y. Yun1, K. J. Baek1, N. S. Kwon1, K.-C. Park3 1Department of Biochemistry, Chung-Ang University College of Medicine2Organic Chemistry Laboratory, Korea Institute of Science & Technology, Seoul3Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea The purpose of this study is to characterize the effects of KHG26792 (3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride), a potential skin whitening agent, on melanin synthesis and identify the underlying mechanism of action. Our data showed that KHG26792 significantly reduced melanin synthesis in a dose-dependent manner. Tyrosinase activity was measured in a cell-free system after the addition of KHG26792. However, KHG26792 did not block tyrosinase directly.