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If a time shift in the peak is equal to the time shift in this regression line should be equal to 1. The onset of the TMS-evoked EMG suppression and the start of the analysis window were defined as the point https://en.wikipedia.org/wiki/Quinapyramine where the ensemble-averaged EMG for the stimulated condition was less than the control EMG for at least 4 ms in a window from 30 to 60 ms after the transcranial stimulus. Short lasting suppressions ( http://www.selleckchem.com/products/MS-275.html the variables in the moving platform protocol (up, levelled, down) a one-way repeated measure analysis of variance (rmANOVA) was used. To test for differences in the TMS protocol (control vs. stimulated) a two-sided Student's t test for paired data was used. All results in the text are given as means �� standard deviation, while the results in Fig. 3 are given http://www.selleckchem.com/products/GDC-0449.html as means �� standard error of the mean for display reasons. The average hopping height of the subjects was 24 �� 3 cm (range 19 to 28 cm). At these hopping heights a prominent early EMG burst within 100 ms after passing the light barrier could always be detected. The passing of the light barrier was followed by touch-down 30 �� 7 ms later, when the platform was in the levelled position. Figure 1 shows the raw and ensemble averaged EMG of a single subject. While SOL shows a distinct early burst, such a burst was less distinct in the ensemble averaged GM, which is in accordance with observations from Voigt et al. (1998). The focus in the analysis was therefore on SOL. It can be observed in the ensemble averages that when the platform was in the up-position both the touch-down and the minimum acceleration of the ankle angle advanced in time, while they were delayed in the down-position. The peak of the early EMG burst shifts in the same direction. Figure 1 shows that the EMG onset relative to the crossing of the light barrier was not different between conditions, which was also observed in the group analysis (1 �� 18 ms; F2,8= 0.490; P= 0.62). The group average of the onset of the SOL EMG in the levelled position is 27 �� 25 ms prior to touch-down and thus well before a reflex component induced by touch-down can contribute to the EMG.