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The evaluation was based on the contribution of the native trans-phytol along with its transformation products cis- and iso-phytol as well as dihydrophytol. Gas chromatography with mass spectrometry operated in the selected ion monitoring mode enabled the detection of these compounds at >0.01?mg/100?g oil or fat. In virgin vegetable oils trans-phytol was the principle phytol related compound while cis-phytol was generally http://www.selleck.cn/products/CAL-101.html not detected at all. Refined vegetable oils contained trans-, cis-, and iso-phytol but no dihydrophytol. Partly and totally hydrogenated vegetable oils contained dihydrophytol if at least 5% hydrogenated fat were present in the oil. The occurrence of cis- and iso-phytol in vegetable oils can be used as indicators for refined oils while the presence of dihydrophytol in vegetable oils is an indication for hydrogenation. Practical applications: The analysis of trans-phytol, cis-phytol, iso-phytol, and dihydrophytol can be used in food control for oil authentication. In this context iso-phytol can serve as an additional marker for refined vegetable oils and dihydrophytol as a marker http://www.selleckchem.com/products/gsk2126458.html for hydrogenated vegetable oils. Native vegetable oils are containing trans-phytol in the unsaponifiable matter. During the refining of vegetable oils measurable amounts of cis-phytol and iso-phytol are formed from trans-phytol. In addition, hydrogenation of oils leads to formation of dihydrophytol from trans-phytol and cis-phytol, http://www.selleckchem.com/products/bay-57-1293.html and these four compounds can be used to authenticate different qualities of vegetable oils. ""PUFA from oil extracted from Nile perch viscera were enriched by selective enzymatic esterification of the free fatty acids (FFA) or by hydrolysis of ethyl esters of the fatty acids from the oil (FA-EE). Quantitative analysis was performed using RP-HPLC coupled to an evaporative light scattering detector (RP-HPLC-ELSD). The lipase from Thermomyces lanuginosus discriminated against docosahexaenoic acid (DHA) most, resulting in the highest DHA/DHA-EE enrichment while lipase from Pseudomonas cepacia discriminated against eicosapentaenoic acid (EPA) most, resulting in the highest EPA/EPA-EE enrichment. The lipases discriminated between DHA and EPA with a higher selectivity when present as ethyl esters (EE) than when in FFA form. Thus when DHA/EPA were enriched to the same level during esterification and hydrolysis reactions, the DHA-EE/EPA-EE recoveries were higher than those of DHA/EPA-FFA. In reactions catalysed by lipase from T. lanuginosus, at 26?mol% DHA/DHA-EE, DHA recovery was 76% while that of DHA-EE was 84%. In reactions catalysed by lipase from P. cepacia, at 11?mol% EPA/EPA-EE, EPA recovery was 79% while that of EPA-EE was 92%.