Hantzsch Reaction as a Method for Spectrofluorimetric Analysis of Penicillin G

A sensitive, selective, and validated spectrofluorimetric method is being described

for the determination of penicillin G. The method utilizes this drug (as a source of the

amine moiety) to react with acetylacetone and formaldehyde through the Hantzsch

reaction. The faint yellow product of dihydropyridine derivatives was measured at an

emission wavelength of 481 nm (after excitation at 416 nm). Linear calibration graphs

were obtained in the range of 0.2-200 μg/mL. The experimental limits of detection and

quantitation were 0.170 and 0.515 μ/mL, respectively. The relative standard deviation

(n=5) at the 100 μg/mL level were 6.11 for intra-day precision and 9.42 for inter-day

precision. The method was successfully applied in the analysis of pharmaceutical

formulation; the recovery was quantitative, and the result obtained agreed with those

obtained by another reported method.

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