Good Usage And Cleaning Practices For Medifuge
Centrifuges are widely used in clinical laboratories for the separation of components. In laboratories that perform biochemical analyzes on body fluids, it is routinely used to separate blood cells from plasma, separate urine sediments, measure the volume fraction of erythrocytes in the blood, and even in DNA extraction processes. Several stages of examinations depend on the action of the medifuge.
There are several types of centrifuges, rotors and specialized tubes to perform centrifugation. You must choose the equipment according to the driving force, rotor, and needs appropriate for the procedure you want to perform. These differences determine the maximum speed of the centrifugation, the temperature control, the capacity of tubes, and the maximum volume of liquid per tube. The first step towards the accuracy of the result is the choice of the best model that meets the needs of your laboratory.
A medifuge is actually utilized to split bits appended in a liquid depending on to fragment measurements as well as quality, viscosity of the channel, and rotating mechanism velocity.
Because it is a very important piece of equipment, in addition to knowing how to handle it correctly, cleaning the equipment is an extremely important factor for your analysis to have a quality result.
CLEANING A Medifuge
The external part of the equipment:
Dip a soft cloth in the detergent water, and with the medifuge closed, clean the entire external area of the equipment, including the panel. Make sure that the equipment is disconnected from the mains.
With a dry cloth, wipe off excess water. The equipment must be completely dry after cleaning.
* Never use volatile chemicals such as alcohol, benzene, etc. Or sponge and any other type of rough material.
The internal part of the equipment:
For cleaning inside the medifuge, remember to remove, according to the instructions for each model, the rotor and the centrifuge chamber. When cleaning the inside of the centrifuge, take care not to get the engine wet. With a dry cloth, wipe off excess water.
Do not forget the cover, the force of the centrifugation can cause sample material to leak.
Centrifuge Chamber and Rotor:
Outside the centrifuge, wash the centrifuge chamber with water, soap, and a soft cloth. With a dry cloth, wipe off excess water.
Remove the adapters from the rotor and wash them with water, soap, and a soft cloth. Don't forget to wash the rotor adapters thoroughly.
For the best quality and precision in the results of your laboratory exams, the cleaning of the centrifuge must always occur after using the equipment. For your safety, before cleaning the equipment, make sure that it is not connected.
BEST PRACTICES IN CENTRIFUGATION
For the centrifugation process to be necessary, it is necessary that some conditions of centrifugation are performed in order to achieve a sequence of work that saves time and the ideal quality of the result without influencing the analysis process. Following the steps below, the risk of errors and failures is reduced in the centrifugation process.
The working surface of the centrifuge must be a firm, flat and non-slip bench to never allow the centrifuge to move.
Proper Rotor Installation:
The rotor of a poorly placed centrifuge can cause errors in the process. The rotor must be carefully fitted with the motor pin. Tighten the screw securely using the Allen key (supplied).
Remember to check that the rotor is firmly adjusted before starting the laboratory routine.
Balancing the centrifuge:
The centrifuge must always be balanced. Thus, for each tube, another tube must be placed on the opposite side with the same weight, balancing the centrifuge.
This process must be done for the entire rotor and every centrifuge, including microcentrifuges and low-speed rotations.
It is worth remembering that a tube with the same volume will not always have the same weight, this will depend on the sample density. In some cases, as in the ultracentrifuge, it will be necessary to weigh the tubes individually.
End of Cycle:
The main consequences of errors are incomplete centrifugation. Never interrupt the spin cycle, and never use your hands as a brake. Wait for the centrifuge to come to a complete stop before removing the tubes.
Operating an uneven centrifuge laboratory equipment might induce significant damages, as well as hurt the operator as well as various other lab workers. The total mass of each tube must be as close as possible- this comes to be progressively significant at extremely high rotating mechanism rates. Balancing masses to the nearby 0.1 gram is suggested, and it is very important to balance tubes by mass, certainly not amount. For example, perform certainly not adjust a sample featuring fluid along with a greater or even reduced thickness than water along with an equivalent quantity of water.