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After washing, the filters were incubated with anti-mouse IgG (1:1000) (Cell Signaling Technology) in TBS-T for 1?h at room temperature and developed to detect specific protein bands using ECL reagent (Amersham Biosciences). The bands on Western blots were quantified using the ImageJ program (National Institutes of Health, Bethesda, MD). The damage was assessed on PAS stained renal tissue sections by scoring tubular dilatation, cast deposition, loss of brush borders, and necrosis in 10 non-overlapping fields (��200) at the corticomedullary junction. Injury was scored on a five-point scale [16]: 0?=?normal, 1?=? http://www.selleckchem.com/products/z-vad-fmk.html 4?=?50�C75%, 5?=?>75%. Data are expressed as means?��?SD. Statistical analysis was performed with unpaired t-test or analysis of variance (anova) for multiple comparisons, followed by Scheffe��s F-test. Differences were considered statistically significant at P? http://www.selleckchem.com/products/ly2157299.html control and EGCG groups. The increase in creatinine in the EGCG group was significantly suppressed compared with the control group at 24?h (control group 1.73?��?0.46?mg/dl, EGCG group 0.81?��?0.23?mg/dl; P? http://www.selleck.cn/products/BIBW2992.html with preserved renal function, kidneys at 72?h after I/R injury in the EGCG group had significantly less renal tubular damage than the control group (Fig.?1b�Cd). We performed immunostaining for cleaved caspase-3 to quantify the number of apoptotic cells in the I/R kidneys. Cleaved caspase-3 was widely expressed among the tubular epithelial cells in the control group, whereas EGCG treatment significantly decreased cleaved caspase-3 expression (Fig.?2). Lipid peroxidation is a well-established mechanism of cellular injury and is used as an indicator of oxidative stress in cells and tissues. Measurement of MDA is widely used as an indicator of lipid peroxidation. The renal tissue MDA content 4?h after reperfusion was significantly increased by I/R injury (47.7?��?9.0?nmol/g tissue) compared with the sham-operated group (23.6?��?2.1?nmol/g tissue; P?